在Fusarium pseudograminearum中对甲可纳的耐药性风险评估和分子基础
Guixiang Li1, Yiwen Li1, Ling Zhang1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, 3 Taicheng Road, Yangling, Shaanxi, 712100, China.
Stress biology
|April 30, 2025
概括
Fusarium pseudograminearum 显示对真菌杀菌剂metconazole产生耐药性的风险很低. 在FpCYP51B基因,特别是M151T的突变和基因过度表达赋予了耐药性,尽管突变者降低了适应性.
科学领域:
- 农业科学 农业科学
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
背景情况:
- 甲子是一种固醇14α-脱甲基化抑制剂 (DMI) 杀菌剂,有效抑制了Fusarium pseudograminearum.
- 了解F. pseudograminearum的耐药性机制和对甲可纳产生耐药性的风险,对于有效的疾病管理至关重要.
研究的目的:
- 为了评估F. pseudograminearum对美特可纳的耐药性风险.
- 确定F. pseudograminearum.中metconazole耐药性的基因和分子机制.
主要方法:
- 确定了对F. pseudograminearum分离物的metconazole的EC50.
- 通过杀菌剂适应生成了耐药突变体,并评估了它们的适应性.
- 研究了与其他DMI杀菌剂的交叉耐药性.
- 在使用分子技术的耐药突变体中识别了突变和基因表达变化.
主要成果:
- 甲可纳对F. pseudograminearum的EC50值很低 (0.0559微克/毫升).
- 耐药突变体在体外表现出降低的适应性.
- 观察到与美芬特里弗卢可纳和特布可纳的积极交叉耐药性.
- 在FpCYP51B中的M151T突变和FpCYP51基因的过度表达被确定为关键的耐药性机制.
结论:
- 由于突变体适应性降低,F. pseudograminearum 患上对美特可纳的耐药性风险较低.
- 在FpCYP51B和FpCYP51基因过度表达中的M151T替代赋予了对甲可纳的耐药性.
- 这些发现提供了对虫类病的杀菌剂耐药性管理策略的见解.
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