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Updated: May 9, 2025
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A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
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在新生儿对败血症的反应中,先天免疫训练
Jaimar C Rincon1,2, Dayuan Wang3, Valerie E Polcz4
1Sepsis and Critical Illness Research Center, Department of Surgery, University of Florida College of Medicine, 1600 SW Archer Road, P.O. Box 100119, Gainesville, FL, 32610 - 0019, USA. jaimar.rincon@surgery.ufl.edu.
Molecular medicine (Cambridge, Mass.)
|April 30, 2025
概括
细菌-卡尔梅特-盖林 (BCG) 疫苗通过重新编程髓状细胞来提高新生儿抗败血症的存活率. 这种活体减弱疫苗增强了训练的免疫力,改善了新生儿对无关感染的反应.
科学领域:
- 免疫学 免疫学 免疫学
- 新生儿研究新生儿研究
- 疫苗学 疫苗学 疫苗学
背景情况:
- 新生儿,特别是早产婴儿,面临着感染的高死亡风险.
- 已知活体减弱疫苗,如BCG,对新生儿的二次感染产生非特异性效应 (NSE).
- 训练有素的免疫和紧急颗粒形成与这些有益的NSE有关,但它们对髓质子集的影响尚不清楚.
研究的目的:
- 为了调查巴西卢斯-卡尔梅特-盖林 (BCG) 疫苗接种如何在新生儿中重新编程骨髓形成.
- 确定BCG诱导的训练免疫对宿主对多微生物败血症反应的影响.
- 为了阐明受早期BCG疫苗接种影响的特定髓质子集和转录变化.
主要方法:
- 在新生儿模型中BCG疫苗接种.
- 流细胞计分析骨髓原生细胞群 (LSK,GMP) 和CD11b+Gr1+细胞.
- 评估骨髓细胞的氧化代谢和T细胞刺激能力.
- 新生儿脊髓细胞的单细胞RNA测序.
主要成果:
- 在多微生物性败血症期间,BCG疫苗接种改善了生存率.
- BCG扩大了关键骨髓原体子集 (LSK,GMP) 和增加了CD11b+Gr1+细胞.
- 疫苗接种增强了髓状细胞的氧化代谢和T细胞增殖能力.
- 单细胞RNA测序揭示了骨髓体子集的广泛转录性重编程,促进中性粒细胞/单细胞成熟,抗菌活性,抑制炎症和骨髓体衍生的抑制细胞.
结论:
- 早期的BCG疫苗接种从根本上重组了新生儿骨髓样板.
- 这种重新编程增强了宿主对随后的多微生物感染的保护性反应.
- 由BCG诱导的训练免疫通过调节骨髓发育提供了针对败血症的显著益处.
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