基因组多样性,种群结构和MALDI-TOF MS对来自发酵和野生环境的Aspergillus oryzae/flavus菌株进行分析
Dong-Hyun Kim1,2, Dong-Chan Kim3, Donggun Seo3
1Korean Agricultural Culture Collection, Agricultural Microbiology Div., National Institute of Agricultural Sciences, RDA, Wanju, 55365, Republic of Korea.
BMC genomics
|April 30, 2025
概括
基因组分析显示,在韩国有不同的Aspergillus oryzae和Aspergillus flavus群. 可以使用基因组和蛋白质组方法从Aspergillus oryzae区分出产生亚亚毒素的菌株,称为Aspergillus flavus.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 食品科学 食品科学 食品科学
背景情况:
- Aspergillus oryzae在韩国大豆发酵中被广泛使用,它来自产生亚毒素的阿斯伯吉卢斯黄菌.
- 了解这些菌株的分类学位置和真菌毒性风险对于食品安全至关重要.
研究的目的:
- 通过基因组分析对韩国Aspergillus oryzae/flavus菌株进行分类.
- 评估真菌毒性潜力,并区分Aspergillus oryzae和Aspergillus flavus之间.
主要方法:
- 人口基因组学被用来分析来自各种韩国环境和全球孤立的菌株.
- 二次代谢物基因集群分析确定了特定组的突变.
- 飞行质谱的矩阵辅助激光脱吸/离子化时间 (MALDI-TOF MS) 用于蛋白质定型.
主要成果:
- 确定了五个不同的基因组 (A-E);在C组 (Aspergillus flavus) 中聚合的非毒性韩国菌株.
- 在C组菌株中,完整的阿弗拉托克辛和环酸基因集群是普遍存在的.
- MALDI-TOF MS通过特定的蛋白质学峰值将C组与其他组区分开来,其中包括与亚毒素代谢相关的峰值.
结论:
- 基因组和MALDI-TOF MS分析有效地确定了韩国阿斯伯菌株的分类学位置.
- 这些方法可以区分Aspergillus oryzae和Aspergillus flavus,这对于评估发酵食品中的真菌毒素风险至关重要.
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