相关实验视频

Updated: May 9, 2025

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer

Published on: October 13, 2023

连楚安齐徐液体阴通过抑制Jagged-1/Notch1信号通路来调节疹性结肠炎的巨细胞极化

Yongli Zhang^1,2, Jiali Liu¹, Dan Zhang¹

¹Department of Anorectal Surgery, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210029, People's Republic of China.

Drug design, development and therapy

|May 1, 2025

概括

连楚安紫徐液体阴道通过抑制M1巨分化和通过Jagged-1/Notch1通路促进M2分化,有效治疗性结肠炎.

科学领域:

胃肠病学胃肠病学
免疫学免疫学免疫学
传统中国医药传统中国医药

背景情况:

性结肠炎 (UC) 是一种慢性炎症性肠病.
传统中医药中的LianChuang ZhiXue液体 (LCZXL) 阴囊在UC患者中显示出临床疗效.
在UC中LCZXL的精确分子机制尚不清楚.

研究的目的:

阐明LCZXL阴囊在治疗UC中的分子机制.
研究Jagged-1/Notch1信号通路和巨细胞极化在LCZXL治疗效果中的作用.

主要方法:

建立了UC小鼠模型,并以不同剂量的LCZXL进行治疗.
评估了疾病活动指数 (DAI),结肠长度和结肠粘膜损伤指数 (CMDI).
免疫组织化学,HE染色,ELISA,PCR,西部斑点和流动细胞测量用于分析M1/M2标记物,细胞因子和Jagged-1/Notch1通路组件.

主要成果:

在UC小鼠中,LCZXL阴囊显著降低了结肠损伤,降低了DAI和CMDI得分.
LCZXL抑制了M1巨标记物 (CD86,iNOS,TNF-α,IL-1β,IL-6) 和促进了M2标记物 (Arg1,CD206,IL-4,IL-10) 的发生.
LCZXL治疗逆转了UC建模诱导的Jagged-1,Notch1和Notch2的上调,并抑制了Jagged-1/Notch1信号通路.

结论:

LCZXL清水有效地改善了性结肠炎中的结肠损伤.
LCZXL通过抑制M1巨分化和促进M2巨分化来发挥其治疗作用.
这些效应通过调节Jagged-1/Notch1信号通路来调节.

关键词:

在Jagged-1/Notch1信号通道中.在 LCZXL 阴囊.M2巨细胞的两极分化性结肠炎是一种

更多相关视频

Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model

Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model

Published on: February 14, 2021

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

Published on: September 18, 2016

相关概念视频

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Drugs for Treatment of Ulcerative Colitis in IBD

Drugs for Treatment of Ulcerative Colitis in IBD

Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...

Drugs for Treatment of Constipation-Predominant IBS

Drugs for Treatment of Constipation-Predominant IBS

Pharmacological therapies for IBS-C are designed to alleviate abdominal discomfort and enhance bowel function. In patients with IBS-C, fiber supplements may help soften stools and decrease straining, but may also lead to increased gas production and bloating. Osmotic laxatives like milk of magnesia are frequently used to soften stools and increase stool frequency in IBS-C patients. In addition, two drugs approved for use in severe IBS-C adult cases are linaclotide (Linzess) and lubiprostone...