相关实验视频
Updated: May 9, 2025

07:56
Measuring Skeletal Muscle Thermogenesis in Mice and Rats
Published on: July 27, 2022
2.8K
用于热生物学的基因编码温度指标.
Tetsuichi Wazawa1, Ryohei Ozaki-Noma1,2, Lu Kai1
1SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Ibaraki, Osaka 567-0047, Japan.
Biophysics and physicobiology
|May 1, 2025
概括
基因编码温度指标 (GETI) 是基于蛋白质的光纳米温度计,用于观察生物样本的温度. 本综述涵盖了热生物学研究的GETI机制,开发和表征.
科学领域:
- 生物物理学的生物物理.
- 分子生物学分子生物学
- 热生物学 热生物学
背景情况:
- 温度是影响分子过程和生物功能的关键物理参数.
- 宏观传感器不足以在微观尺度 (例如细胞,有机体) 上测量温度.
- 光纳米热仪为生物系统中非侵入性温度测量提供了解决方案.
研究的目的:
- 审查基于蛋白质的光纳米温度计,也称为遗传编码温度指标 (GETI).
- 提供GETI感知机制,测量方法和开发策略的概述.
- 提出表征标准并讨论热生物学中的未来前景.
主要方法:
- 关于遗传编码温度指标 (GETI) 发表文献的审查.
- 对温度传感机制和光显微镜技术的分析.
- 检查用于GETI开发的蛋白质工程方法.
主要成果:
- 各种基于蛋白质的光纳米温度计 (GETI) 的全面概述.
- 详细解释温度传感原理和测量方法.
- 识别用于GETI构建的独特蛋白质工程技术.
结论:
- GETI是用于在生物样本中进行非侵入性温度监测的宝贵工具.
- 标准化表征对于可靠的GETI开发和应用至关重要.
- 进一步的GETI研究将推动热生物学领域的发展.
相关概念视频
Equipments Used to Measure Body Temperature
901
Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
901
Body Temperature
831
The body's temperature, measured in degrees, is determined by the balance between heat production and dissipation to the surrounding environment. For instance, if exercising vigorously, the body will produce more heat, causing sweat and dissipating that heat. Despite extreme environmental conditions and physical exertion, the human temperature-control system maintains a constant core body temperature (the temperature of deep tissues, which are the tissues located beneath the skin and other...
831
Thermosensation
30.2K
Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
30.2K
Reporter Genes
11.0K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.0K
Assessing Body Temperature - Oral
689
Here are the steps to accurately measure oral temperature using an electronic thermometer:
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
689

