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Updated: May 12, 2025

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

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Published on: June 23, 2013

纤维细胞信号对巨细胞偏振的影响

Samuel T LoPresti¹, Mangesh M Kulkarni¹, Dana R Julian¹

¹Department of Bioengineering, University of Pittsburgh Swanson School of Engineering, Pittsburgh, Pennsylvania; McGowan Institute of Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania.

The American journal of pathology

|May 1, 2025

概括

纤维细胞分泌的产物会影响巨细胞的行为,促进炎症和纤维化. 激活的纤维细胞显著影响巨细胞的两极分化和功能,突出显示它们在纤维性疾病中的作用.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
病理学病理学病理学

背景情况:

纤维化疾病是全球主要的健康问题.
巨细胞-纤维细胞相互作用在纤维化发育中至关重要.
了解这种交叉通话是开发抗纤维化疗法的关键.

研究的目的:

研究纤维细胞分泌的产物如何影响巨细胞的两极分化和功能.
为了确定暴露在纤维细胞产物中的时间的影响.
阐明纤维细胞-巨细胞交叉在纤维化中的作用.

主要方法:

巨细胞子组 (M0,M1,M2) 被暴露在静止或激活纤维细胞的条件介质中.
暴露发生在巨细胞极化之前,期间或之后.
宏细胞功能通过细胞酶,氧化,阿基纳酶活性和细胞因子阵列进行评估.

主要成果:

活性纤维细胞介质在M0和M2巨细胞中显著增加了阿尔金酶-1.
激活的纤维细胞介质在所有巨细胞子集中调节了IL-1β和IL-6.
在用活性纤维细胞介质治疗的M1巨中,瘤缩因子-α增加.

结论:

纤维细胞,特别是激活的纤维细胞,对巨细胞产生显著的促炎和促纤维细胞效应.
纤维细胞-巨细胞相互作用的时间会影响巨细胞的反应.
这项研究提供了关于驱动纤维化的复杂机制的见解.

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