开发生物基的蛋废弃物载体,用于R. oryzae的脂酶固定化
Ayda Torchi1, Hanen Ghamgui1, Emna Moujehed1
1Laboratory of Biochemistry and Enzymatic Engineering of Lipases, Biological Engineering Department, National Engineering School of Sfax (ENIS), University of Sfax, 3038, Tunisia.
概括
这项研究成功地将R. oryzae脂酶 (ROL) 固定在无膜卵 (MFE) 上,达到99.55%的产量. 在循环经济应用中,ROL@MFE证明了生物催化剂的稳定性和潜力.
科学领域:
- 生物催化剂是一种生物催化剂.
- 生物材料是一种生物材料.
- 循环经济是一个循环经济.
背景情况:
- 可持续的生物废弃物管理对循环经济战略至关重要,生物废弃物矩阵上的酶固定化是一个重大挑战.
- 蛋是一种易于获得的生物废物,具有作为酶固定矩阵的潜力.
研究的目的:
- 为了将R. oryzae脂酶 (ROL) 固定在没有膜的蛋 (MFE) 上.
- 描述固定酶 (ROL@MFE) 并评估其稳定性和生物催化剂的潜力.
主要方法:
- 使用优化的条件 (15794 IU脂酶,30分钟,380rpm) 进行酶固定.
- 吸附异热模型 (弗朗德利希,兰格穆尔,粒子内部扩散).
- 使用泽塔电位进行表征,粒子大小分析,衍射模式,电导率,EDS和SEM.
主要成果:
- 在MFE上实现了ROL99.55%的固定收益率.
- 吸附数据最适合弗洛伊德利希和兰格穆尔模型,其动力学由粒子内部扩散描述.
- 描述证实了成功的固定,没有晶体结构扰动,揭示了弱离子键和增加的C/O含量.
结论:
- 该ROL@MFE表现出卓越的稳定性和固定效率.
- 该方法提供了一种可持续的方法,用于生物废物利用和酶固定化,用于生物催化.
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