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N6 - - 甲基氨酸的修改稳定了与酸盐饥饿反应相关的mRNA,用于植物适应营养缺乏压力

Kai Liu¹, Xiaojia Wang¹, Jingyi Wang¹

¹State Key Laboratory of Plant Environmental Resilience, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.

Nature communications

|May 1, 2025

概括

植物中的N-甲基氨酸 (m6A) 修饰是由酸盐饥饿诱导的,有助于调节基因表达. 这项研究揭示了m6A增强了关键应激反应基因的稳定性,有助于营养缺乏适应.

科学领域:

植物分子生物学植物分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
营养物质应激反应的应激反应

背景情况:

N6-甲基氨酸 (m6A) 是一种关键的mRNA修饰,可以在压力下调节基因表达.
m6A在植物营养缺乏反应,特别是酸盐 (Pi) 饥饿中的作用尚不清楚.

研究的目的:

调查m6A修饰在Arabidopsis thaliana对无机正酸盐 (Pi) 缺乏条件的反应中的作用.
阐明m6A影响酸盐饥饿反应 (PSR) 的机制.

主要方法:

在正常条件和Pi缺陷条件下对高置信度m6A修饰的全转录组概况.
识别m6A调节器,包括酸盐化反应1 (PHR1) 和m6A写入器 (MTA,MTB).
在对m6A修饰的反应中分析mRNA衰变速率和稳定性.

主要成果:

饥饿显著诱导广泛的m6A修饰,由PHR1及其家族成员介导.
PI饥饿诱导 (PSI) m6A修饰发生在PSR相关的mRNA上,包括PHR1,并增强其稳定性.
m6A修改通过稳定关键PSR相关的转录来促进系统PSR信号传输,形成一个积极的反循环.

结论:

PSI-m6A的修改对于提高参与酸盐饥饿反应的特定mRNA的稳定性至关重要.
m6A通过在转录后调节基因表达,在植物适应营养缺乏方面发挥着至关重要的作用.
这项研究揭示了一种新的机制,将m6A修饰与植物中营养应激信号联系起来.

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