相关实验视频
Updated: Jun 14, 2025

08:06
Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
Published on: February 14, 2016
7.9K
组装和激活Hook3 - KIF1C复杂依赖运输机械的分子基础
Hye Seon Lee1, Daseuli Yu2, Kyoung Eun Baek3
1Disease Target Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Korea.
EMBO reports
|May 1, 2025
概括
子微管连接蛋白3 (Hook3) 作为基因素家族成员1C (KIF1C) 和dynein的支架,促进前级货物运输. 结构和功能分析显示,Hook3-KIF1C复合体的形成对这一过程至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 结构生物学是结构生物学.
背景情况:
- 微管相关的货物运输对细胞功能至关重要,涉及诸如kinesin和dyneins之类的运动蛋白.
- 子微管连接蛋白3 (Hook3) 作为货物适配器,将运动蛋白与货物连接起来.
- 众所周知,Hook3可以将素家族成员1C (KIF1C) 和dynein都用于双向运输.
研究的目的:
- 阐明Hook3通过哪些结构和功能机制来调解KIF1C依赖的前级货物运输.
- 为了研究Hook3,KIF1C和PTPN21之间的相互作用.
- 确定Hook3-KIF1C复合体在蜂货物运输中的作用.
主要方法:
- 进行X射线晶体学以确定Hook3-KIF1C复合物的结构.
- 同免疫沉检验HEK293T细胞中的蛋白相互作用.
- 在RPE1细胞中进行定位突变和功能测试,以评估该复合体在前进运输中的作用.
主要成果:
- 确定了Hook3 ((553-624) -KIF1C ((714-809) 复合物的晶体结构.
- 验证了Hook3,KIF1C和PTPN21之间的相互作用.
- 基于结构的突变分析证实了Hook3-KIF1C复合物的必要性和充分性,用于全长蛋白相互作用和前级传输.
结论:
- 3-KIF1C复合体形成是启动依赖于3-和KIF1C的前层货物运输的关键步骤.
- 了解这个复合体,可以了解基于微管的运动蛋白活动的调节.
- 这项研究为更深入地了解货物运输机械组装奠定了基础.
相关概念视频
Coat Assembly and GTPases
3.5K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.5K
The Movement of Organelles and Vesicles
4.4K
In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
4.4K
Protein Complex Assembly
10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K
Microtubule Associated Motor Proteins
7.7K
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
7.7K
Assembly of Signaling Complexes
5.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.7K
Assembly of Cytoskeletal Filaments
18.6K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
18.6K

