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模块通过抑制OsPrx30介导的ROS扫除,调节米的抗菌病菌的抵抗力

Wenya Xie^1,2, Xinru Li¹, Xuanyu Yue²

¹National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.

Journal of agricultural and food chemistry

|May 2, 2025

概括

米 WRKY 转录因子调节免疫力. OsWRKY53与OsVQ32相互作用,形成一个抑制OsPrx30表达的复合物,通过调节活性氧物种影响细菌炎症耐药性.

科学领域:

植物分子生物学植物分子生物学
植物病理学植物病理学
生物化学生物化学

背景情况:

WRKY转录因子是植物免疫力的关键调节者.
之前已经证明OsWRKY53通过影响细胞壁厚度来降低大米对细菌病菌的抵抗力.

研究的目的:

为了阐明免疫中OsWRKY53的分子机制.
在OsMPK4-OsVQ32级联的背景下,研究OsWRKY53和OsVQ32之间的相互作用.

主要方法:

酵母二杂交测定以确认蛋白质与蛋白质的相互作用.
电泳运动转移试验 (EMSA) 来评估DNA结合活性.
定量实时PCR (qRT-PCR) 用于分析基因表达.
测量过氧化酶活性和过氧化含量.

主要成果:

OsWRKY53与OsVQ32进行物理相互作用,在OsMPK4-OsVQ32级联的下游运行.
OsVQ32-OsWRKY53复合体抑制了OsPrx30转录,这是大米细菌病菌耐药性的负调节器.
通过OsMPK4对OsVQ32的酸化增强了该复合物的抑制活性.
OsWRKY53的过度表达减少了反应性氧物种 (ROS) 清理,而突变体则表现出相反的效果.

结论:

由OsMPK4介导的化调节的OsVQ32-OsWRKY53复合体调节了大米细菌病菌的耐药性.
这种调节通过抑制OsPrx30表达和随后对反应性氧物种清理的影响而发生.

关键词:

在 OsVQ32 的位置上.这就是ROSOS ROS.这是一种细菌性疫病.在OSWRKY53中使用米米饭米饭米饭米饭.

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