在细菌中工程古老的膜跨越脂质GDGT生物合成:对早期生命膜转换的影响
Huahui Chen1, Fengfeng Zheng1, Xi Feng1
1Department of Ocean Science and Engineering Southern University of Science and Technology Shenzhen China.
细菌可以结合简单的archaeal膜脂质,如 digeranylgeranylglycerol (DGGGOH) 和archaeol,没有显著的伤害. 然而,复杂的脂质,如甘二甲基甘四 (GDGT) 是有害的,这表明它们是独特的古生物.
科学领域:
- 细胞生物学 细胞生物学
- 进化生物学是进化的生物学.
- 生物化学 生物化学
背景情况:
- 人们认为,真核生物是古生物-细菌仿真体.
- 鉴于古生物学和细菌膜脂质的差异,真核细胞膜的起源仍然是一个难以理解的难题.
- 考古 - 细菌混合膜是真核生成的潜在中间体,但尚未观察到.
研究的目的:
- 研究考古-细菌混合膜的可行性和后果.
- 了解在真核生成过程中膜脂质组成的进化含义.
主要方法:
- 重建了Escherichia coli*中古细胞膜脂质生物合成途径.
- 创建了三种产生不同数量的古老脂质的细菌菌株 (DGGGOH,archaeol,GDGT).
- 分析了细菌对纳入的考古脂质的生理反应.
主要成果:
- 细菌可以耐受简单的古老脂质 (DGGGOH,archaeol) 以最小的健身成本.
- 复杂的古物脂质,特别是GDGT的结合,诱导了严重的细胞应激反应 (SOS反应,丝状,溶解).
- 在GDGT生物合成过程中观察到ATP的积累.
结论:
- 简单的archaeal膜脂质可以集成到细菌膜,支持混合膜在早期细胞进化和eukaryogenesis的假设.
- 像GDGT这样的复杂的古生物学脂质可能是一种古生物学创新,因为它们对细菌的有害作用.
- 这项研究提供了实验性的洞察力,了解了在真核生物起源期间膜进化的挑战.
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