pituitary folliculo-stellate 细胞调节瘤血管结构和细胞外基质组成在实验性乳腺瘤中
S A Valla1, A Chimento2, G Demarchi1
1Centro de Investigaciones Básicas y Aplicadas, Universidad Nacional Del Noroeste de la Provincia de Buenos Aires, Junín, Buenos Aires, Argentina; Centro de Investigaciones y Transferencia Del Noroeste de La Provincia de Buenos Aires (CITNOBA) - UNNOBA-UNSAdA-CONICET, Pergamino, Buenos Aires, Argentina.
概括
毛囊星状细胞 (FSCs) 调节 pituitary 瘤的生长. 虽然它们可以刺激瘤细胞增殖,但FSCs也抑制瘤血管生成,缺氧和细胞外矩阵重塑,揭示了双重调节作用.
科学领域:
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
- 在瘤学瘤学.
背景情况:
- 毛囊星状细胞 (FSCs) 是 pituitary 细胞功能的关键调节者.
- FSCs在垂体瘤发育中的作用尚不清楚.
- 了解FSC参与对于开发向疗法至关重要.
研究的目的:
- 研究FSCs在垂体瘤形成,激素分泌,血管化和细胞外矩阵 (ECM) 改造中的作用.
- 阐明FSCs在调节瘤生长和进展中的双重功能.
主要方法:
- 在免疫缺陷小鼠中使用GH3细胞和FSC (TtT/GF) 生成乳糖体热性垂体瘤模型.
- 在体外共同培养GH3和TTT/GF细胞以评估增殖和分泌作用.
- 对瘤生长,激素合成,血管化 (VEGF,bFGF),缺氧标志物 (Hif1a) 和ECM组件的分析.
主要成果:
- 同时注射GH3和TTT/GF细胞最初加速了瘤的发病,但后来抑制了瘤的生长和益生菌素的合成.
- 在实验室中,FSC对GH3细胞表现出直接的线粒发生效应.
- GH3瘤显示缺氧增加和血管化减少,而同时注射的瘤显示增强了ECM沉积.
- 隔离的TTT/GF细胞用原和bFGF形成纤维质,而不是瘤.
结论:
- FSCs作为垂体瘤生长的双重调节者.
- FSCs既对瘤细胞具有线粒体效应,也对血管新生,缺氧和ECM重塑具有瘤限制作用.
- 这些发现突出了FSCs和垂体瘤发生之间的复杂相互作用.
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