Stm1 调节 Ifh1 活动,揭示了核糖体生物发生和核糖体休眠之间的交叉声
Eliana Bianco1, Martina Bonassera2, Federico Uliana1
1Department of Biology, Institute of Biochemistry, Eidgenössische Technische Hochschule (ETH), 8093 Zürich, Switzerland.
Molecular cell
|May 2, 2025
概括
Tom1 (Stm1) 抑制剂与酵母菌中的核糖体保护和生物发生有联系. 它激活Ifh1,控制核糖体蛋白基因转录,其功能对细胞生长和营养感应至关重要.
科学领域:
- 分子和细胞生物学分子和细胞生物学
- 酵母遗传学 酵母遗传学
- 核糖体生物发生和恒常性.
背景情况:
- 营养素的可用性通过平衡核糖体生物发生和降解来影响细胞生长.
- 核糖体休眠因子保护核糖体免受饥饿期间的降解.
- 这些过程之间的相互作用对于维持细胞平衡至关重要.
研究的目的:
- 确定连接核糖体保护和生物发生的分子链接.
- 为了阐明Tom1 (Stm1) 抑制剂在酵母菌Saccharomyces cerevisiae中的作用.
- 为了了解Stm1如何调节核糖体恒温,以应对营养的可用性.
主要方法:
- 确定Stm1作为一个关键的调节器.
- 对Stm1定位到核细胞的分析.
- 通过其内在无序区域 (IDR) 调查Stm1与前核糖体,RNA和Ifh1的相互作用.
主要成果:
- Stm1 作为一个分子环节,激活 Ifh1,这是核糖体蛋白质基因的转录激活器.
- Stm1的C端IDR对于其与Ifh1的相互作用和细胞生长至关重要,尽管它对于核糖体保护是不可或缺的.
- 在营养缺乏的情况下,IDR的酸化会破坏Stm1-Ifh1的相互作用,调节核糖体生物发生.
结论:
- 一个涉及Stm1的新型分子途径感知了营养物质的可用性,以调整核糖体丰富度.
- 通过Stm1和Ifh1调节的核糖体平衡,对酵母生理学至关重要.
- 这些发现对理解健康和疾病中的核糖体平衡有意义.
相关概念视频
Regulation of the Unfolded Protein Response
2.3K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.3K
Regulation of Expression at Multiple Steps
843
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
843
Riboswitches
8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
Regulation of Expression Occurs at Multiple Steps
21.9K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
21.9K
Chromatin Structure Regulates pre-mRNA Processing
6.8K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
6.8K
mRNA Stability and Gene Expression
5.5K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.5K


