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鉴定一个塔加4-表皮酶的特征,并优化其表达,以便从果糖中有效地生物转化D-塔加

Shenxi Zhao¹, Jiajun Chen¹, Yuhan Wei¹

¹State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.

International journal of biological macromolecules

|May 2, 2025

概括

研究人员发现了一种新型酶DRJ43-T4Ease,它可以有效地将d-果糖转化为功能甜味剂D-塔加. 这一发现为工业规模的D-塔加生物生产提供了一个有前途的途径.

科学领域:

生物技术是生物技术.
酵素工程是什么? 酶工程是什么
工业微生物学工业微生物学

背景情况:

D-Tagatose是一种功能性甜味剂,具有有益的生理作用.
提高D-塔加的效率和成本效益的生产方法越来越重要.
随时可用的资源的生物转化是甜味剂生产的关键研究领域.

研究的目的:

确定和描述一种能够合成D-塔加的新型酶.
优化已识别的酶的表达和活性,用于工业应用.
通过全细胞生物转化证明D-塔加生产的可行性.

主要方法:

生物信息分析用于识别来自Thermoprotei古生物的假定塔加-4-epimerase (DRJ43-T4Ease).
生物化学特征DRJ43-T4Ease,包括最佳条件 (pH,温度,辅因子) 和运动参数.
酶工程和优化翻译启动序列,以增强大肠杆菌的表达.
使用工程化大肠杆菌菌株的全细胞生物转化实验,将d-果糖转化为D-塔加.

主要成果:

DRJ43-T4Ease证明了d-果糖转化为D-塔加的有效转化,在pH7.5和90°C时在Ni2+的存在下具有最佳活性.
该酶表现出高热稳定性,化温度 (Tm) 为105.9°C,并且在高温下具有显著的半衰期.
使用100g/L的d-果糖,工程化大肠杆菌菌株 (EcDRJ43-T4Ease-M1) 实现了98.4U/L的体积活性和17.2%的转化效率.

结论:

DRJ43-T4Ease是一种高效和热稳定的塔加-4-酶,具有产生D-塔加的潜力.
使用工程化大肠杆菌的优化全细胞生物转化为工业规模的D-塔加合成提供了可行的策略.
这项研究提出了一种有前途的生物技术方法,用于可持续生产功能甜味剂D-塔加.

关键词:

生物合成生物合成塔加-4-表皮酶的使用开始翻译开始翻译 d-果糖是一种糖.d-塔加托糖的使用.

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