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相关概念视频

RNA-seq03:21

RNA-seq

9.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: May 9, 2025

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
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通过集成的RNA捕获和库准备,TIRE-seq简化了转录学.

Piper O'Keeffe1,2, Yasmin Nouri1,2, Hui Shi Saw1,2

  • 1The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade VIC, Melbourne, VIC, 3052, Australia.

Scientific reports
|May 2, 2025
PubMed
概括

旋捕获集成RNA表达序列 (TIRE-seq) 通过将mRNA净化整合到图书馆准备中来简化基因表达研究. 这种方法可以降低成本和时间,同时提高大规模研究的数据质量.

关键词:
提取 提取 提取高吞吐率的高吞吐量.这是一个RNARNARNARNARNA.文字转录学 (Transcriptomics) 是一个学科.

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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相关实验视频

Last Updated: May 9, 2025

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物技术是生物技术.

背景情况:

  • RNA测序 (RNA-seq) 对于理解基因表达至关重要,但传统上涉及耗时的RNA提取.
  • 现有的方法可能会导致样本丢失和复杂的研究工作流程的成本增加.

研究的目的:

  • 开发一种精简的RNA测序方法,不需要单独提取RNA.
  • 为了提高效率,减少样本损失,提高基因表达分析中的数据质量.

主要方法:

  • 开发了TIRE-seq (Turbocapture集成RNA表达序列),将mRNA净化整合到图书馆准备中.
  • 将TIRE-seq与使用原始细胞溶解物的Prime-seq协议进行比较.
  • 应用TIRE-seq来分析受刺激的人类T细胞中的基因表达,分化小鼠树突细胞,以及用temozolomide治疗的神经圈.

主要成果:

  • 与使用原始细胞溶解体的Prime-seq相比,TIRE-seq显示出更高的测序效率.
  • 成功捕获激活的T细胞的转录变化,并确定树突细胞分化中的关键基因.
  • 在患者衍生的神经圈中,鉴定了对temozolomide的反应中差异表达的基因和途径.

结论:

  • TIRE-seq为基因表达研究提供了一种简化,具有成本效益的工作流.
  • 该方法提高了测序效率和数据质量,使其适合大规模研究.
  • TIRE-seq是多功能,适用于各种生物系统和研究问题.