相关实验视频
Updated: May 12, 2025
10:24
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
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一个精心策划的细菌和考古16S rRNA基因口腔序列数据集
Lara Vázquez-González1,2, Alba Regueira-Iglesias2,3, Carlos Balsa-Castro1,2,3
1Centro Singular de Investigación en Tecnoloxías Intelixentes (CiTIUS), Universidade de Santiago de Compostela, Rúa de Jenaro de la Fuente Domínguez, E15782, Santiago de Compostela, Spain.
Scientific data
|May 2, 2025
概括
本研究介绍了16S rRNA基因口腔序列数据集 (16SGOSeq),详细介绍了口腔微生物中的16S rRNA基因拷贝数. 本资源改善了人类口腔微生物组研究中的微生物丰度估计.
科学领域:
- 微生物学 微生物学
- 生物信息学是一种生物信息学.
- 基因组学就是基因组学.
背景情况:
- 基因内16S rRNA基因拷贝数量因物种和环境而异.
- 准确的基因拷贝数数据对于微生物丰富度估计技术,如PCR和测序至关重要.
- 基因拷贝数量的差异可能导致微生物种群的低估或高估.
研究的目的:
- 创建一个16S rRNA基因拷贝数和口腔 prokaryotes 的变异的专业数据库.
- 为准确量化人类口腔中的微生物丰富性提供可靠的资源.
- 支持临床微生物学,生物信息学和微生物生态学的未来研究.
主要方法:
- 从完整的细菌和古生物基因组中提取16S rRNA基因序列.
- 在口腔微生物群中识别序列变异.
- 编制了16S rRNA基因口腔序列数据集 (16SGOSeq).
主要成果:
- 16SGOSeq数据集包括3,192个细菌和191个考古学完整的口腔基因组.
- 提供了关于16S rRNA基因拷贝数和序列变异的详细信息.
- 开发了一个构建口腔特定基因复制号数据库的管道.
结论:
- 16SGOSeq数据集为口腔微生物组研究提供了关键的,利基特定的数据.
- 该资源提高了使用基因计数方法估计微生物丰度的准确性.
- 开发的管道和数据集是相关领域的研究人员有价值的工具.
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