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商用PRRSV减弱疫苗可以成为一种潜在有效的活体三价值疫苗载体

Yang Li¹, Yumiao Wang¹, Xiuxiu Pei²

¹State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.

Applied microbiology and biotechnology

|May 2, 2025

概括

研究人员制造了一种修改后的猪生殖和呼吸系统综合征病毒 (PRRSV) 疫苗,该疫苗表达了外来光蛋白. 这种重组PRRSV成功诱导了对PRRSV和小猪中的异物蛋白的抗体,显示出新的多价值疫苗的潜力.

科学领域:

兽医病毒学兽医病毒学
疫苗开发疫苗开发
分子生物学分子生物学

背景情况:

猪生殖和呼吸系统综合征病毒 (PRRSV) 由于免疫抑制和对二次感染的易感性,在猪业中造成重大经济损失.
开发多价值疫苗对于同时控制多种病原体和改善猪健康至关重要.
商用PRRSV疫苗为新型疫苗策略提供潜在的病毒载体.

研究的目的:

评估使用商业高度致病性PRRSV (HP-PRRSV) 改性活病毒 (MLV) 疫苗菌株作为病毒载体的可行性.
构建一种表达两种外来光蛋白 (EGFP和mCherry) 的重组PRRSV,以便在三价疫苗中潜在使用.
评估表达异源蛋白质的复合PRRSV的稳定性和免疫性.

主要方法:

通过嵌入光蛋白 (mCherry和EGFP) 的基因,设计了一种HP-PRRSV MLV疫苗菌株 (GD) 的传染性克隆.
构建了一个能够同时表达EGFP和mCherry的重组PRRSV (rPRRSV-mCherry-EGFP).
在MARC-145细胞中通过串行传递评估了复合病毒的稳定性,并在小猪身上评估了其免疫性.

主要成果:

在感染MARC-145细胞中,rPRRSV-mCherry-EGFP成功地表达了EGFP和mCherry蛋白,表达水平与感染相关.
再组合病毒表现出稳定性,在至少10代传递后保持外源蛋白质的表达.
用复合rPRRSV-mCherry-EGFP免疫的小猪对PRRSV,EGFP和mCherry产生了抗体.

结论:

修改后的HP-PRRSV MLV疫苗菌株显示出作为开发多价值疫苗的病毒载体具有前景.
EGFP和mCherry的成功联合表达和免疫性表明,有可能嵌入来自多种病原体的基因.
这种方法为构建有效的三价值疫苗提供了基础,可以提供针对PRRSV和其他疾病的交叉保护.

关键词:

一个传染性克隆.插入插入方式标记物基因是一个标记物基因.多价值疫苗的使用情况 PRRSVV 在这个问题上.疫苗的载体是疫苗的载体.

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