在免疫细胞疗法制造中优化病毒转导:关键的过程设计考虑因素
1Bioprocessing Technology Institute BTI, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, Singapore, 138668, Singapore. liu_dan@bti.a-star.edu.sg.
Journal of translational medicine
|May 2, 2025
概括
病毒转导是免疫细胞疗法制造的关键,使基因传递成为可能. 本综述分析了优化这一复杂过程的挑战和创新,以获得可扩展,安全和有效的疗法.
科学领域:
- 生物技术是生物技术.
- 免疫学 免疫学 免疫学
- 细胞治疗制造业 细胞治疗制造业
背景情况:
- 免疫细胞疗法为各种疾病提供了革命性的治疗选择.
- 病毒转导是将治疗基因送入免疫细胞的关键制造步骤.
- 当前的制造工艺在优化和可扩展性方面面临着挑战.
研究的目的:
- 在免疫细胞疗法制造中提供病毒转导的全面分析.
- 突出开发安全,有效和可扩展的生产方法的关键设计考虑因素.
- 检查技术挑战,并探索免疫细胞转导的未来创新.
主要方法:
- 在免疫细胞治疗中对病毒转导技术的文献综述.
- 对流程优化和可扩展性的设计考虑的分析.
- 检查当前的技术障碍和新兴的创新.
主要成果:
- 病毒转导复杂性对免疫细胞疗法制造提出了重大挑战.
- 关键的设计考虑对于实现安全,有效和可扩展的生产至关重要.
- 技术进步对于克服当前的局限性至关重要.
结论:
- 优化病毒转导对于推进免疫细胞治疗制造至关重要.
- 通过创新解决当前的挑战,将促进下一代疗法的发展.
- 对可扩展和高效的传导方法进行进一步的研究是有必要的.
相关概念视频
01:27Upstream Processing
95
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
95
01:23Vaccine Production
120
Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
120