在水中生物合成RiPP基因集群的生物信息探索
Anna Pasinato1,2, Garima Singh3,4,5
1Department of Biology, University of Padova, Via U. Bassi, 58/B, Padova, 35121, Italy.
Fungal biology and biotechnology
|May 2, 2025
概括
液体形成的真菌拥有庞大的,尚未探索的收藏Ribosomally合成和翻译后修饰的 (RiPPs) 生物合成基因集群 (BGCs). 这项研究揭示了广泛的,独特的RiPP BGCs,包括 dikaritin同类物,这表明这些共生真菌中产生了新的真菌毒素.
科学领域:
- 菌类学和生物化学 菌类学和生物化学
- 基因组学和生物信息学
- 同生相互作用的交互.
背景情况:
- 核糖体合成和翻译后改性 (RiPPs) 是具有治疗潜力的真菌代谢物.
- RiPPs是由生物合成基因集群 (BGCs) 编码的,通常参与真菌防御.
- 在共生形成真菌 (LFF) 中的RiPP BGC景观仍然在很大程度上没有特征.
研究的目的:
- 进行首次对形形成真菌中的RiPP BGC进行全面调查.
- 探索Lecanoromycetes中的RiPP BGCs的多样性和进化关系.
- 识别新的RiPP BGC及其潜在功能,包括菌毒素的产生.
主要方法:
- 111个形形成的真菌基因组的基因组挖掘.
- 基因推断和基因网络重建已识别的RiPP BGCs.
- 与已知的RiPP BGCs和保存的基因 (HXXHC) 的比较分析.
主要成果:
- 发现了987个RiPPBGC,占LFF生物合成多样性的17%,远高于预期.
- 在Parmeliaceae中鉴定了两种保存的RiPP BGCs,同类于ustiloxin BGCs,含有二甲合成基因 (R1家族) 或新型细菌类辅助基因 (R2家族).
- 在Lecanoromycetes中广泛分布的假定卡卡里类同类,由签名基因中保存的HXXHC动机表明.
结论:
- 菌形成的真菌拥有丰富多样的RiPP BGC,包括新型类型.
- 在Parmeliaceae中保存的两种RiPP BGC被假定与菌毒素 (dikaritins) 生产有关.
- 这项研究揭示了化真菌中假定真菌毒素相关的BGCs的普遍存在,扩大了我们对共生中的真菌二次代谢的理解.
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