第三类合成酶为psilocybe代谢组添加循环和线性类
Nick Zschoche1, Sebastian Schober2, Karl Walther2
1Friedrich-Schiller-Universität Jena: Friedrich-Schiller-Universitat Jena, Pharmaceutical Microbiology, GERMANY.
概括
魔法产生的不仅仅是精神素. 这项研究在Psilocybe cubensis中确定了四种新的基合成酶 (CubB-CubE),扩大了我们对真菌二次代谢物的理解.
科学领域:
- 菌类学 菌类学是指菌类学.
- 自然产品化学 自然产品化学
- 酶学 是一种酶学.
背景情况:
- Psilocybe 是以 psilocybin 闻名的,但它也含有各种生物活性分子的酶.
- Psilocybe 种类的二次代谢组在很大程度上是未被探索的.
- 酸合成酶的基因表达在果实体和体之间有所不同.
研究的目的:
- 来自Psilocybe cubensis. cubensis的四种新型基合成酶 (CubB-CubE) 的特征.
- 调查CubB-CubE的体外和体内酶活性.
- 为了解Psilocybe属的更广泛的二次代谢组做出贡献.
主要方法:
- 异质酶生产和体外产物形成试验.
- 在Aspergillus niger中转基因表达用于体内表征.
- 气色谱-质谱 (GC-MS) 用于产品的识别和分析.
主要成果:
- CubB作为一个 (3R,6E) - - - - - - 尼罗利多尔合成酶起作用.
- CubC是一种多产品酶,产生β-,β-,α-和β-.
- 在CubD和Cube中,主要产生的物质是素.
- 在P. cubensis组织中检测到α-(3R,6E) -(-) - - 尼罗利多尔.
结论:
- 来自P. cubensis的四种新型基合成酶 (CubB-CubE) 已被功能性地描述.
- 这项研究揭示了Psilocybe菌中基的多样性比以前知道的要大.
- 这些发现有助于了解真菌的二次新陈代谢和Psilocybe属的酶多样性.
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