在Phaeobacter中,DmdA独立的滞后阶段缩短在压力条件下抑制细菌
Delia A Narváez-Barragán1, Martin Sperfeld1, Einat Segev1
1Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel.
The FEBS journal
|May 3, 2025
概括
细菌通过使用DMSP等化合物的甲基组来加速生长. 一个关键的酶,贝他因-同氨酸S-甲基转移酶 (Bmt),作为脱甲基酶和氨酸合成酶,有助于在压力下适应.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细菌利用二甲基硫 propionate (DMSP) 等化合物的甲基组,通过 metionin 循环缩短滞后阶段.
- 在这个过程中,氨酸合成酶的确切作用仍然不完全理解.
研究的目的:
- 为了研究特定的氨酸合成酶,涉及到滞后阶段缩短在Phaeobacter inhibens.
- 阐明贝他因-同氨酸S-甲基转移酶 (Bmt) 类复合物的功能,以应对DMSP和压力条件.
主要方法:
- 使用了转录学,遗传学和生物化学分析.
- 研究了一种类似于MetH的复合物,涉及贝他因-同氨酸S-甲基转移酶 (bmt),科巴胺结合蛋白 (cbp) 和PGA1_c16040.
- 用删除突变物和功能测定来确定酶活性.
主要成果:
- 在对DMSP的反应中删除bmt受损的滞后相缩短.
- Bmt从DMSP和贝他因中直接合成氨酸,独立于THF或可巴胺.
- 即使没有DmdA在压力下,也发生了滞后相缩短,随着对DMSP和贝他因的反应增加了bmt表达.
结论:
- Bmt 作为去甲基酶和甲氨酸合成酶,特别是在压力下,提供有效的甲基同化策略.
- 确定了一种用于 metionin 合成的新型应激反应途径.
- 在细菌适应过程中,Bmt通过加速滞后期,特别是在压力下,发挥着至关重要的作用.
相关概念视频
Bacterial Growth Curve
6.0K
The bacterial growth curve is a fundamental concept in microbiology that describes the dynamics of bacterial population growth in a closed system with controlled environmental conditions, such as temperature and nutrient availability. This curve is divided into four distinct phases: lag, log (exponential), stationary, and death phases, each reflecting a unique stage of bacterial adaptation and growth. During the lag phase, bacteria acclimate to their surroundings by synthesizing essential...
6.0K
Stringent Response in E. coli
533
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
533
Other Stress Responses in Bacteria
593
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
593
Bacterial Phylum Proteobacteria
1.1K
Proteobacteria, one of the largest and most diverse bacterial phyla, encompasses a wide range of Gram-negative bacteria distinguished by their outer membrane composed of lipopolysaccharides. These microorganisms exhibit various metabolic capabilities, including phototrophy, chemolithotrophy, and heterotrophy, and thrive in diverse environments from soil to aquatic systems and host-associated niches. The phylum is divided into six classes: Alphaproteobacteria, Betaproteobacteria,...
1.1K
Deep Sea Microbial Ecology
55
The deep ocean and its underlying sediments represent vast, largely unexplored microbial habitats that extend far beyond the sunlit photic zone. The photic (euphotic) zone typically spans the upper ~100–200 meters of pelagic waters in the open ocean, but its depth varies geographically and seasonally, where sufficient light supports photosynthetic life. Below this lies the deep sea, spanning roughly 1000–6000 meters (bathypelagic to abyssal zones), with deeper hadal trenches...
55
Regulation of Bacterial Virulence
81
Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...
81


