菌类塑造细菌的基因组进化,即使没有主要的生长表型
Emily E Putnam1,2, Robert May1, Nina Freeman1
1Tufts University, Department of Biology, Medford, MA, 20155.
The ISME journal
|May 3, 2025
概括
微妙的真菌相互作用显著改变细菌基因表达和基因组进化,即使没有重大生长变化. 这些微生物动态影响生态和进化路径.
科学领域:
- 微生物生态学 微生物生态学
- 微生物进化 微生物进化
- 基因组学就是基因组学.
背景情况:
- 微生物相互作用研究往往侧重于大的生长差异和短期结果.
- 微妙的相互作用,缺乏明显的表型,可以显著塑造微生物生态和进化.
研究的目的:
- 研究真菌物种 (Penicillium camemberti,Geotrichum candidum) 对细菌Pseudomonas carnis LP.基因表达和基因组演变的影响.
- 了解微妙的真菌-细菌相互作用如何影响微生物社区动态和进化轨迹.
主要方法:
- 使用奶酪皮的模型微生物组.
- 在与真菌共同培养时,分析了P. carnis LP的全球基因表达变化.
- 单独和每个真菌一起演变的P. carnis LP,观察基因组突变.
- 进行了代谢和健身实验.
主要成果:
- 在P. carnis LP基因组的4-40%中,真菌伴侣诱导了差异性基因表达,尽管生长效应有限.
- 突变经常发生在调节的全球调节者,二次代谢产物和运动性中.
- 观察到ntrB基因的特定突变,受真菌伴侣的影响.
- 通过P. camemberti释放的氨基酸抵消了ntrB突变的优势.
结论:
- 菌物种可以深刻影响细菌转录组和基因组进化,独立于显著的短期生长效应.
- 微妙的微生物相互作用是生态动态和长期进化过程的关键驱动因素.
- 特定的真菌伴侣决定了相互作用细菌中基因组进化的性质和程度.
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