相关实验视频

Updated: May 17, 2025

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

库拉里和诺-库拉里抑制NLRP3炎症酶激活,并调节对性结肠炎的巨分化

Wanyu Li¹, Yadi Li¹, Yongyi Qiu¹

¹School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.

International immunopharmacology

|May 3, 2025

概括

库拉里和诺-库拉里通过抑制NLRP3炎症酶和平衡M1/M2巨分化来缓解性结肠炎 (UC). 这些化合物为UC患者提供了潜在的新疗法.

科学领域:

免疫学免疫学免疫学
胃肠病学胃肠病学
药理学药理学是指药理学的学科.

背景情况:

类似NOD的受体蛋白3 (NLRP3) 炎症酶激活驱动炎症和巨细胞失衡在性结肠炎 (UC).
了解NLRP3炎症酶调节和巨细胞极化对于开发有效的UC疗法至关重要.

研究的目的:

在UC的小鼠模型中研究Kurarinone和Nor-kurarinone的治疗潜力.
阐明这些化合物调节NLRP3炎症酶激活和巨细胞极化的机制.

主要方法:

性结肠炎 (UC) 在小鼠中被诱导使用硫酸 (DSS).
用Kurarinone和Nor-kurarinone治疗小鼠以评估治疗效果.
在体外研究中,使用LPS和ATP/Nigericin刺激的THP-1细胞来模拟NLRP3炎症酶激活.
研究了作用机制,重点关注NLRP3/ASC相互作用和巨细胞两极分化.

主要成果:

库拉里和诺库拉里治疗显著缓解了UC症状,包括体重减轻和疾病活性.
这些化合物减少了结肠中的NLRP3炎症酶的形成,并在UC小鼠中调节了巨细胞极化.
在体外,Kurarinone和Nor-kurarinone破坏了NLRP3 / ASC相互作用,抑制了炎细胞组合和调节巨细胞两极分化.
治疗导致肠道巨细胞的M1极化减少,促进M1/M2平衡.

结论:

库拉里和诺-库拉里有效抑制NLRP3炎症酶组合和激活.
这些化合物重新平衡肠道巨细胞两极分化,从M1转向M2表型.
库拉里和诺-库拉里是治疗性结肠炎的有前途的新型治疗药物.

关键词:

库拉里诺是一个人.巨细胞是一个巨细胞.在NLRP3中,NLRP3是NLRP3中的一个.诺-库拉里诺没有一个.性结肠炎是一种

更多相关视频

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea

Published on: December 23, 2022

相关概念视频

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

Drugs for Treatment of Ulcerative Colitis in IBD

Drugs for Treatment of Ulcerative Colitis in IBD

Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...

Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids

Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids

Glucocorticoids, a class of anti-inflammatory drugs, are pivotal in treating moderate to severe Crohn's disease by inducing remission. They exhibit their anti-inflammatory action by inhibiting the production of inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1, and chemokines like IL-8. In addition, they reduce the expression of inflammatory cell adhesion molecules and inhibit gene transcription of nitric oxide synthase, phospholipase A2, cyclooxygenase-2...

Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF

Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF

Tumor Necrosis Factor (TNF), a proinflammatory cytokine, contributes significantly to the inflammation seen in Crohn's disease. It exists as soluble TNF and membrane-bound TNF, with actions mediated through TNF receptors (TNFR). TNFR activation leads to the release of proinflammatory cytokines, T-cell activation, collagen production, and leukocyte migration, all contributing to inflammation in Crohn's disease. Anti-TNF monoclonal antibodies, namely infliximab (Remicade), adalimumab...

Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents

Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents

Crohn's disease is an inflammatory bowel disorder marked by chronic inflammation of the GI tract. Various treatment strategies for Crohn's disease are employed, such as immunomodulatory agents, glucocorticoids, and biologics or anti-TNF therapy. Azathioprine (Imuran), a commonly used immunomodulatory drug for Crohn's disease, is converted in the body to mercaptopurine, which inhibits purine biosynthesis and cell proliferation. Both are utilized in severe cases of Inflammatory Bowel...

Inflammatory Response

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...