对表达的CHO细胞的特征分析 MERS-CoV RBD-Fcc
Mashal M Almutairi1, Yi-Lin Chen2, Jungsoon Lee2
1Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
概括
由于正在进行的MERS-CoV循环,需要MERS疫苗. 这项研究特征了一种有前途的MERS-CoV RBD-Fc疫苗抗原,发现它在广泛的pH范围内稳定,这对疫苗开发至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 蛋白质工程是指蛋白质工程.
背景情况:
- 中东呼吸系统综合征冠状病毒 (MERS-CoV) 在驼中全球传播,构成动物感染风险.
- 人类MERS-CoV感染需要开发有效的疫苗.
- 以前开发的与人类Fc (RBD-Fc) 融合的MERS-CoV受体结合域显示出作为疫苗抗原的前景.
研究的目的:
- 描述由重组CHO细胞系产生的MERS-CoV RBD-Fc蛋白质.
- 为了评估纯化的MERS-CoV RBD-Fc抗原的完整性,身份和稳定性.
- 为了确定MERS-CoV RBD-Fc蛋白稳定性的最佳缓冲条件.
主要方法:
- 产生一个稳定的重组中国仓鼠卵巢 (CHO) 细胞系表达MERS-CoV RBD-Fc.
- 使用内部分析试验来评估蛋白质的特性,完整性和身份.
- 进行了缓冲选实验,以确定蛋白质稳定性的最佳配方.
主要成果:
- MERS-CoV RBD-Fc蛋白在广泛的pH范围 (3.5-9.5) 中表现出结构和功能完整性.
- 一个中性至基本的pH环境被确定为增强蛋白质稳定性的最佳环境.
- 最佳条件降低了表面水性,并改善了MERS-CoV RBD-Fc蛋白的体稳定性.
结论:
- 有特征的MERS-CoV RBD-Fc蛋白质对疫苗开发具有有利的特性.
- 了解产品特性对于推进MERS疫苗制造和疗效至关重要.
- 这些发现为MERS疫苗候选人的进展提供了有价值的数据.
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