在人类牙上使用拉曼光谱法进行先进的非破坏性性别确定
Raphael Hug1, Anna E Wood1, Frank J Rühli1
1Institute of Evolutionary Medicine, University of Zürich, Winterthurerstrasse 190, 8057, Zürich, Switzerland.
Scientific reports
|May 3, 2025
概括
拉曼光谱法使用牙面膜以非破坏性的方式估计生物性别. 该方法根据光谱特征准确区分男性和女性个体,为法医和考古学分析提供了宝贵的工具.
科学领域:
- 法医人类学 法医人类学
- 生物分子分析
- 考古学科学 考古学科学
背景情况:
- 在法医科学,人类学和考古学中,对分析人类骨遗骸至关重要.
- 目前用于性别估计的方法对于某些样本类型可能是破坏性的或不切实际的.
- 牙面膜为非破坏性分析提供了持久且潜在的信息基质.
研究的目的:
- 调查拉曼光谱作为一种非破坏性方法,以使用完整的人类牙结膜来估计生物性别的有效性.
- 为了识别牙质内性别特定的光谱标记.
- 根据这些光谱差异,开发一种可靠的性别确定模型.
主要方法:
- 使用拉曼光谱学分析47个现代人的88颗人类牙.
- 应用直角局部最小平方差分分析 (OPLS-DA) 来识别光谱模式.
- 开发一个包含关键拉曼转移波数 (373,1182,1600 cm-1) 的逻辑回归模型.
主要成果:
- OPLS-DA模型表现出高性能 (R2Y() =0.943,Q2Y() =0.895) 的情况.
- 后勤回归模型实现了高精度:ROC-AUC为0.98,灵敏度为0.87,特异性为0.94.
- 确定了牙面膜的性别特异的光谱差异,可能与阿米洛根因蛋白质变异有关.
结论:
- 拉曼光谱法提供了一种快速,非破坏性和可靠的方法,用于从人类牙面膜中估计生物性别.
- 这种技术在考古学和法医学上特别有价值,因为样品的完整性至关重要.
- 这些发现表明,质中微妙的分子组成差异可以作为可靠的性别歧视者.
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