从非原核和单原核的胚芽细胞中发展的胚芽细胞的使用可以在PGT-SR:一项回顾性队列研究中考虑
Jingzhen Wang1,2,3,4,5,6,7, Hongqiang Xie1,2,3,4,5,6,7, Yang Zou1,2,3,4,5,6,7
1State Key Laboratory of Reproductive Medicine and Offspring Health, Center for Reproductive Medicine, Cheeloo College of Medicine, Institute of Women, Children and Reproductive Health, Shandong University, 157 Jingliu Road, Jinan, 250012, Shandong, China.
BMC pregnancy and childbirth
|May 3, 2025
概括
零和一个前核 (0PN和1PN) 胚胎通常不建议用于结构重组 (PGT-SR) 周期的植入前遗传测试. 然而,如果只有很少或没有常规的两核前 (2PN) 胚胎可用,则可以考虑它们,优先考虑0PN胚胎.
科学领域:
- 生殖医学 生殖医学
- 胚胎学 胚胎学
- 遗传学 遗传学 是一个
背景情况:
- 目前的协议往往不包括零 (0PN) 或一个 (1PN) 亲核的密室.
- 接受结构重组 (PGT-SR) 的植入前遗传测试的患者需要大量的胚胎,原因是形积分率高.
研究的目的:
- 研究0PN和1PN胚胎在PGT-SR周期中的生存能力和潜力.
- 为了确定这些胚胎是否可以增加结构重组的患者的euploid胚胎的产量.
主要方法:
- 在422个辅助生殖周期中对4,868个囊胚进行了回顾性分析.
- 在54个周期中,对来自0PN和1PN胚胎的芽细胞进行了PGT-SR.
- 在343个胚胎 (0PN,1PN和2PN) 上进行了全面的基因组哈普洛型化.
主要成果:
- 与2PN胚胎相比,0PN和1PN胚胎的芽细胞发育率明显较低.
- 0PN的欧普洛伊德率与2PN没有显著差异,但1PN的比率明显低.
- 包括0PN/1PN胚胎显示冷,可转移和转移胚胎的数量没有显著增加.
结论:
- 染色体异常并没有增加异常受精率.
- 不建议将0PN和1PN胚胎常规包含在PGT-SR中.
- 只在2PN胚胎不足时考虑0PN/1PN胚胎,优先考虑0PN,并使用全基因组测试.
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