circPOLD1及其结合蛋白YBX1在宫癌发生中的生物标志物潜力
Lu Zhao1, Xin Chen2, Yanan Zhang1,3
1Department of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, No.1 Xueshi Road, Hangzhou, 310006, China.
Journal of translational medicine
|May 3, 2025
概括
新的生物标志物circPOLD1和YBX1显示出早期宫癌检测的前景. 它们在查中的联合使用显著提高了准确性,为干预和减少癌症负担提供了新的途径.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 宫癌查依赖于细胞学和HPV测试,这些测试有局限性.
- 循环RNAs (circRNAs) 是稳定的,特定于组织的分子,具有作为生物标记物的潜力.
- 在宫癌发生过程中,circRNAs的作用和它们在早期检测中的临床实用性尚未得到充分研究.
研究的目的:
- 为了研究宫癌各个阶段的动态circRNA表达.
- 确定新的circRNA生物标志物,以提高宫癌的早期检测.
- 探索circPOLD1在宫癌发生过程中的机械作用.
主要方法:
- 在正常的,高度状细胞内皮损伤 (HSIL) 和宫状细胞癌 (CSCC) 组织上进行circRNA测序.
- 功能性试验评估circPOLD1的致癌潜力和与YBX1.1.的相互作用.
- 用BaseScope,免疫组织化学 (IHC) 和ROC曲线分析进行临床验证和血清中circPOLD1的诊断性能评估.
主要成果:
- CircPOLD1的表达逐渐从正常增加到HSIL和CSCC.
- CircPOLD1作为一种瘤基因,通过YBX1激活AKT/mTOR/HIF-1α通路,以促进糖解驱动的瘤发生.
- 一个circPOLD1/YBX1面板显示了高的诊断准确性 (AUC为LSIL+的0.951,HSIL+的0.817);血清circPOLD1水平与疾病阶段相关.
结论:
- CircPOLD1和YBX1协同驱动子宫癌发生,具有特定阶段的表达.
- 联合检测circPOLD1和YBX1显著提高了宫癌查和监测的准确性.
- 贝斯科普和IHC验证表明风险分层和早期干预的直接转化潜力.
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