储存温度对甲固定型氨酸嵌入组织样本中核酸质量的影响
Yoichiro Okubo1, Nagisa Toyama1, Rika Kasajima2
1Department of Pathology, Kanagawa Cancer Center, Yokohama, Japan.
Annals of diagnostic pathology
|May 4, 2025
概括
在-20°C或更低的温度下存储甲固化嵌 (FFPE) 组织,可以保持基因组分析的核酸质量. 室温储存导致降解,影响分子测试可靠性.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 病理学 病理学 病理学
背景情况:
- 在基因组分析中,固定甲嵌入式 (FFPE) 组织至关重要.
- 存储不当会降解核酸,影响分子测试的准确性.
研究的目的:
- 为了评估不同储存温度对FFPE样本中核酸质量的影响.
- 确定最佳的储存条件,以保持FFPE组织完整性,以便进行长期分子分析.
主要方法:
- 来自四个瘤病例的FFPE组织块被存储在不同的温度下 (18°C,4°C,-20°C,-80°C,-150°C).
- 核酸质量 (DNA和RNA) 在12个月内使用DIN,S/L Ct比,RIN和DV200等指标进行评估.
- 还评估了重复的冷解周期的影响.
主要成果:
- 在18°C和4°C保存的样本显示了依赖时间的核酸降解.
- 在-20°C或更低的温度下存储,在12个月内保持稳定的DNA和RNA质量.
- 即使在较低温度下重复结解周期时,也没有观察到任何显著的降解.
结论:
- 储存在-20°C是有效的,以保持FFPE组织中的核酸完整性.
- 建议冷藏用于存档FFPE样本,以便将来进行分子分析.
- 研究结果为优化组织存档策略提供了实际指导.
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