通过工程酶改进C4衍生物的生物合成
Zeyao Chen1, Changxi Zhang2, Bing Xu2
1Zhejiang University, Hangzhou, 310058, PR China; Westlake University, Hangzhou, 310030, PR China.
Metabolic engineering
|May 4, 2025
概括
研究人员利用大肠杆菌 (Escherichia coli) 来将回收塑料中的乙烯基醇 (EG) 转化为1,4-butanediol和酸盐等有价值的化学物质. 这种合成生物学方法为利用塑料废物进行生物制造提供了一个可持续的途径.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 生物技术是生物技术.
背景情况:
- 由聚乙烯二甲 (PET) 酶降解而产生的乙烯基醇 (EG) 是可持续生物制造的潜在原料.
- 目前生产C4化合物的方法,如1,4-butanediol (1,4-BDO) 和1,2,4-butanetriol (1,2,4-BTO),通常依赖于石化来源.
- 需要有效的生物途径来将C2基质转化为高价值的C4化学物质.
研究的目的:
- 在大肠杆菌中开发一种新的代谢途径,用于从C2基质 (如糖和EG) 中生物合成C4化合物.
- 通过定向进化来提高关键酶的催化效率,以改善基质同化.
- 证明PET衍生EG的直接利用用于生产有价值的化学品,有助于塑料废物回收利用.
主要方法:
- 通过一种新的代谢途径进行工程化大肠杆菌 (大肠杆菌).
- 采用定向进化来优化来自Cupriavidus necator的β-ketoacyl thiolase B (CnBktB) 酶,识别了L89S突变.
- 利用增长合选平台进行酶进化.
- 集成了一个上游模块,用于将EG转化为糖.
- 使用糖,葡萄糖和PET衍生的EG作为基质进行发酵实验.
主要成果:
- 使用糖和葡萄糖,获得的产量标位为1,4-BDO>200 mg/L,1,2,4-BTO>266 mg/L和9,22 g/L.
- 在CnBktB的L89S突变体中,Glycolyl-CoA和Acetyl-CoA同化的催化效率得到了提高.
- 直接利用PET衍生的EG,产生11.4g/L糖酸盐,转化效率为93%.
- 展示了一个可扩展的途径,用于将C2前体转化为高价值的C4化合物.
结论:
- 成功建立了一个合成生物学平台,用于从C2基质生产1,4-BDO,1,2,4-BTO和酸盐.
- 改造的大肠杆菌菌株和优化的酶为可持续生物制造提供了基础.
- 这项工作将塑料废物回收利用与生物经济战略相结合,为减少环境影响提供了一个有希望的方法.
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