在新鲜与玻璃化卵子中的受精和胚胎发育与来自非阻塞性亚精子症男性的精子
Christopher P Moutos1, Anjile An2, Zev Rosenwaks1
1Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medical College, New York, New York.
Fertility and sterility
|May 4, 2025
概括
新鲜或玻璃化卵子在与微剖析丸精子提取 (mTESE) 精子一起使用时产生类似的早期胚胎发育. 这一发现在各种比较组中是一致的,突出显示了卵细胞数量对成功受精的重要性.
科学领域:
- 生殖医学 生殖医学
- 胚胎学 胚胎学
- 不孕症研究 不孕症研究
背景情况:
- 严重的男性因子不孕症在辅助生殖技术方面带来了挑战.
- 微切割丸精子提取 (mTESE) 能够为非阻塞性阿佐精子症的男性提取精子.
- 卵细胞冷保存 (玻璃化) 是一种成熟的技术,但它对mTESE精子的影响需要检查.
研究的目的:
- 为了比较早期胚胎发育使用新鲜与玻璃化卵子与mTESE精子受精.
- 为了评估受精和第3天的胚胎质量在夫妇接受细胞内精子注射 (ICSI) 严重的男性因子不孕症.
主要方法:
- 对于使用mTESE精子进行ICSI的夫妇进行了回顾性队列研究.
- 新鲜卵细胞与玻璃化卵细胞的比较.
- 评估受精率 (2-pronuclei) 和优质的第3天胚胎比例.
- 分析包括自我匹配,整个人口和年龄匹配的比较.
主要成果:
- 在新鲜卵细胞和玻璃化卵细胞之间没有观察到受精率或优质的第3天胚胎发育的显著差异.
- 在自我匹配的比较中,中位数受精率为48% (新鲜) 和33% (玻璃化).
- 3日优质胚胎的比例为25% (新鲜) 和21% (玻璃化) 每个受精卵细胞.
结论:
- 玻璃化卵子是使用mTESE精子进行ICSI的可行选择,产生与新鲜卵子相似的胚胎发育.
- 这一群体的低受精率强调了无论存储方法如何,都需要足够的卵细胞获取.
- 这些发现支持使用玻璃化卵子细胞治疗非阻塞性亚精子症男性的生育治疗.
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