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在与agouti相关的神经元中的GPR40信号介导脂肪偏好
Yueping Ge1, Huidong Zhan1, Shanshan Wu1
1Department of Endocrinology, Shandong Provincial Hospital & Medical Integration and Practice Center, Shandong University, Jinan, Shandong 250021, China; Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, China; Key Laboratory of Endocrine Glucose & Lipids Metabolism and Brain Aging, Ministry of Education, Shandong Key Laboratory of Endocrinology and Lipid Metabolism; Shandong Institute of Endocrine and Metabolic Diseases, Shandong Clinical Research Center of Diabetes and Metabolic Diseases, Shandong Prevention and Control Engineering Laboratory of Endocrine and Metabolic Diseases, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China.
在Agouti相关 (AgRP) 神经元中的脂肪酸受体GPR40调节脂肪偏好. 在AgRP神经元中删除GPR40会减少脂肪摄入量,并改变养行为.
科学领域:
- 神经科学是一个神经科学.
- 代谢过程中的代谢.
- 分子生物学分子生物学
背景情况:
- 脂肪偏好受到多个系统中的脂肪酸受体的影响.
- 中枢神经系统脂肪酸受体,如GPR40在调节脂肪偏好的特定作用仍然在很大程度上未知.
- 弧形核 (ARC) 中的Agouti相关 (AgRP) 神经元是食欲和能量平衡的关键调节者.
研究的目的:
- 研究中长链脂肪酸受体GPR40在调节脂肪偏好的作用.
- 为了确定下丘脑弧形核 (ARC) 的阿古蒂相关 (AgRP) 神经元中的GPR40是否调解脂肪偏好.
- 阐明涉及的潜在神经和分子机制.
主要方法:
- 产生AgRP神经元特异的Gpr40淘汰小鼠.
- 行为测试来评估饮食脂肪偏好.
- 在饥饿后进行代谢分析,测量AgRP神经元活动,量化AgRP和神经Y (NPY) 表达水平.
主要成果:
- 针对AgRP神经元的Gpr40淘汰赛小鼠对食脂肪的偏好有所减少.
- 在AgRP神经元中Gpr40的缺失导致AgRP神经元活动增加和饥饿后的AgRP/NPY水平升高.
- 这些变化与减少脂肪摄入量和增加碳水化合物摄入量有关,没有观察到行为异常.
结论:
- 在下丘脑AgRP神经元内传递GPR40信号对于调节脂肪偏好至关重要.
- GPR40调节AgRP神经元活动和AgRP和NPY的表达,从而影响饮食选择.
- 这项研究确定GPR40是脂肪偏好的中央调节中的新型分子参与者.
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