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隔离具有聚基甲酸降解潜力的海洋细菌,并优化酶生产

Antika Boondaeng¹, Chanaporn Trakunjae¹, Pilanee Vaithanomsat¹

¹Kasetsart Agricultural and Agro-Industrial Product Improvement Institute, Kasetsart University, Bangkok, 10900, Thailand.

Scientific reports

|May 4, 2025

概括

确定了能够降解聚酸酸盐 (PHAs) 的海洋细菌. 一个分离物,SS1-2,显示PHA脱聚合酶生产的高潜力,为塑料废物生物降解提供了一个有前途的解决方案.

科学领域:

环境微生物学环境微生物学
生物技术是生物技术.
聚合物科学聚合物科学

背景情况:

由于塑料的分解速度缓慢,塑料的积累对环境构成重大威胁.
聚酸酸 (PHAs) 是可生物降解的塑料,但需要有效的降解方法.
海洋环境是具有塑料降解能力的微生物的潜在来源.

研究的目的:

隔离和描述能够降解PHA的一种类型聚R) -3-基黄油酸 (P3HB) 的海洋细菌.
从这些细菌中识别和优化细胞外PHA脱聚合酶的产生.
评估这些酶在PHA生物降解中的潜在应用.

主要方法:

收集了海洋碎片以选降解P3HB的细菌.
六个细菌分离体显示了P(3HB) 降解,由清晰区域表示.
使用统计方法优化SS1-2分离物,包括响应表面方法 (RSM).
16S rRNA基因测序用于细菌识别.

主要成果:

确定了六种能够降解P(3HB) 的海洋细菌分离物.
孤立的SS1-2呈现出最高的降解指数 (1.44).
使用RSM的优化表明,在0.5%的基底负荷和0.5%的NH4Cl.下,最大的P(3HB) 脱聚合酶产生.
分离物SS1-2被确定为Pseudooceanicola antarcticus CGMCC 1.12662与97.81%的相似性.

结论:

海洋细菌,特别是 Pseudooceanicola antarcticus SS1-2,对于PHA生物降解具有显著的潜力.
优化的条件增强了P(3HB) 脱聚合酶的产生,这表明工业应用.
已确定的PHA脱聚合酶是进一步研究酶动力学和塑料废物管理的有希望的候选者.

关键词:

生物塑料的生物塑料脱聚合酶酶是一种脱聚合酶.细胞外酶是一种细胞外酶.表面响应方法的方法.

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