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适应性和分泌效率之间的反向关系在一个グラム阳性细菌

Kamilla Wiull¹, Morten Kjos¹, Vincent G H Eijsink¹

¹Faculty of Chemistry, Biotechnology and Food Science, NMBU-Norwegian University of Life Sciences, Ås 1433, Norway.

|May 5, 2025

概括

乳植物菌 (Lactiplantibacillus plantarum) 可以在其表面显示抗原,但这会影响细菌健康. 信号的特性极大地平衡了蛋白质分泌效率与细菌的健康状况,这对于优化输送系统至关重要.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

包括Lactiplantibacillus plantarum在内的乳酸细菌正在探索粘膜抗原的传递.
使用LPxTG的异质蛋白质的表面显示可以损害细菌的健康.
优化蛋白质分泌和定对于有效的基于细菌的输送系统至关重要.

研究的目的:

调查信号对异质蛋白质分泌和L. plantarum细菌适应性的影响.
了解分泌效率与细菌活力之间的权衡背后的机制.
确定影响蛋白质转位和表面显示的关键信号特性.

主要方法:

对11个表达HaloTag记者蛋白质的重组L. plantarum菌株进行分析,其中具有不同的信号和一个共同的LPxTG.
基于光联体的标签,用于同时检测细胞内和细胞外折叠的HaloTag.
对分泌效率,蛋白质折叠和细菌生长之间的相关性分析.
信号的突变研究,以证实观察到的相关性.

主要成果:

信号的变化显著影响了细胞内与细胞外折叠的HaloTag的比例.
在细菌健康 (生长) 和分泌效率之间观察到一个反向相关性.
更高的分泌效率,通过增加表面显示而表明,与细菌生长受损有关.
信号的疏水性与分泌效率正相关,但与细菌适应性负相关.

结论:

信号在调节蛋白质分泌和细菌活力之间的平衡方面发挥着至关重要的作用.
分泌过载,可能是由特定的信号特性引起的,可能会损害细菌的生长.
了解信号的功能对于为蛋白质分泌和表面显示应用设计强大的L. plantarum菌株至关重要.

关键词:

在HaloTag中使用HaloTag.这是一种LPxTG蛋白质.细菌健康细菌健康异质蛋白质分泌的异质蛋白质分泌信号是一种信号.

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