3D生物打印厚厚的肝脏结构与血管网络作为一个生理相关的体外器官模型
Young-Wook Moon1, Timothy Dobroski1, Kelsey Willson1
1Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, 27157, United States.
Materials today. Bio
|May 5, 2025
概括
研究人员开发了厚厚的,血管化的肝脏组织结构,使用了新的状腺设计和3D打印. 这些工程组织在体外30天内保持了细胞活力和肝脏特异性功能.
科学领域:
- 生物技术是生物技术.
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 血管化是创造可行的,厚厚的工程器官的关键障碍.
- 现有的方法很难实现足够的血管网络用于代谢组织工程.
研究的目的:
- 开发一种新的方法来制造厚厚的,血管化的代谢组织结构.
- 创建一个生理上相关的人类肝脏组织体外模型.
主要方法:
- 使用数字光投影 (DLP) 3D打印,使用PEG/GelMA生物墨.
- 将人类肝细胞 (HepG2) 和内皮细胞 (ECs) 纳入状腺架构结构.
- 输液培养长达30天.
主要成果:
- 在30天内构建维护尺寸和>85%的细胞活力.
- 免疫光检测证实了肝细胞和EC存在,以及血管通道的EC内.
- 肝细胞在与人类相比的水平上产生了白蛋白和白血素.
结论:
- 在试验室中成功地设计了厚厚的,血管化的人类肝脏组织.
- 陀螺设计促进了均的流动和剪切应力,支持组织功能.
- 这代表了创建功能性体外血管化器官模型的重大进步.
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