香烟烟雾调节LEF1-AS1的甲基化水平,并阻碍其表达:一项实验研究
Bader O Almutairi1, Ahmed Rady1, Bashayer S Aljuhani1
1Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Tobacco induced diseases
|May 5, 2025
概括
香烟烟雾 (CS) 增加了LEF1-AS1基因促进体的DNA甲基化,并显著降低了其在内皮细胞中的表达. 这项研究突出了CS CS.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 癌症研究 癌症研究
背景情况:
- 香烟烟雾 (CS) 是一种已知的致癌物,它影响遗传调节和表观遗传修饰,包括DNA甲基化.
- 长非编码RNAs (lncRNAs),如淋巴细胞增强剂结合因子1反感应RNA1 (LEF1-AS1),与各种癌症有关.
- 在几种癌症中,LEF1-AS1的过度表达,但在白血病中是低调的,这表明它在瘤发生过程中起着复杂的作用.
研究的目的:
- 为了研究香烟烟雾对LEF1-AS1促进体区域DNA甲基化的影响.
- 为了研究香烟烟雾对内皮细胞中LEF1-AS1表达的作用.
- 用公共数据库分析各种癌症类型中的LEF1-AS1表达模式.
主要方法:
- 在64名男性受试者 (32名吸烟者,32名非吸烟者) 的样本上使用双硫酸盐处理和甲基化特异性PCR进行DNA甲基化分析.
- 在人静脉内皮细胞 (HUVECs) 中进行LEF1-AS1表达分析,暴露在CS中3小时和6小时.
- 利用GTEx和GENT2数据库进行癌症和组织表达分析,包括超生存和功能丰富分析.
主要成果:
- 与非吸烟者相比,在吸烟者中观察到LEF1-AS1促进者DNA甲基化显著增加 (19.8%).
- CS暴露导致HUVEC中LEF1-AS1表达的显著减少 (45%在3小时,83%在6小时).
- 在不同瘤类型中,LEF1-AS1的表达有所不同,一些癌症如肺癌和脑癌表现出过度表达;超生存分析表明潜在的风险增加,但缺乏强烈的统计意义.
结论:
- 暴露在香烟烟雾中会改变LEF1-AS1 DNA甲基化状态.
- 对CS的暴露抑制了内皮细胞中LEF1-AS1的表达.
- 这些发现表明,CS诱导的LEF1-AS1表观遗传修饰在癌症发展中可能发挥作用.
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