地瓜结核死病毒编码的运动蛋白NSm与GTP和ATP结合
Rishi Raj1, Abhay Kumar2, H S Savithri3
1Department of Botany, School of Life Science, Mahatma Gandhi Central University, Motihari, Bihar 845401 India.
3 Biotech
|May 5, 2025
概括
核桃芽瘤病毒 (GBNV) 非结构蛋白-m (NSm) 与ATP和GTP结合,其中GTP是首选的配体. 这种相互作用,可能由非典型的Walker A动图介导,对于GBNV的移动和细胞间贩运至关重要.
科学领域:
- 植物病毒学 植物病毒学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 核桃芽死亡病毒 (GBNV) 是一种具有三部分负感RNA基因组的tospovirus.
- MRNA编码非结构蛋白-m (NSm),这是托斯波病毒中已知的运动蛋白.
- 了解NSm的分子相互作用是破译病毒病原和宿主-病原动态的关键.
研究的目的:
- 为了研究GBNV NSm.的核酸结合特性.
- 确定NSm的潜在连接体和结合机制.
- 探索NSm-核酸相互作用在病毒运动中的功能影响.
主要方法:
- 使用[γ-32P]ATP检测GBNV NSm-ATP复合形成的紫外线交联测定.
- 用ATP和GTP合树脂进行糖树脂结合试验.
- 内在光灭研究,以评估NSm-核酸相互作用.
- GTPase活性测定和初步序列分析用于假定的NTP结合动机.
主要成果:
- GBNV NSm直接与ATP和GTP结合,对GTP有偏好 (结合常数为3μM).
- NSm与ATP形成了不同的Mg2+依赖和Mg2+独立的复合体.
- 内在光火证实了NTP和dNTP的结合,GTP的最大火.
- 在NSm中确定了假定非典型的Walker A动图 (aa 51-58和267-274),在tospovirus中保存.
结论:
- 这是第一份报告,证明NSm在任何托斯波病毒物种中都能结合ATP和GTP.
- 鉴定的GTP和ATP结合能力,以及GTP酶活性,表明在能源依赖过程中发挥了作用.
- NSm和GTP之间的相互作用可能对GBNV细胞间贩运和通过等离子体的移动至关重要.
相关概念视频
Intracellular Movement of Viruses and Bacteria
2.7K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.7K
Regulation of Nuclear Protein Sorting
2.3K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.3K
Nuclear Export
3.5K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
3.5K
Nuclear Localization Signals and Import
5.4K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of 2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
5.4K
Nuclear Protein Sorting
4.4K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.4K
Nonsense-mediated mRNA Decay
10.4K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.4K


