在微瓦特级别的多光子显微镜通过增益管理的非线性放大和脉冲采集
Katarzyna Kunio1, Grzegorz Soboń1, Jakub Bogusławski1
1Laser & Fiber Electronics Group, Faculty of Electronics, Photonics and Microsystems, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.
Biomedical optics express
|May 5, 2025
概括
我们开发了一种用于多光子显微镜的紧纤维激光系统. 这种多功能激光器能够在非常低的激发功率下进行高分辨率成像,增强实验灵活性并推动生物研究.
科学领域:
- 生物光子学和成像技术
- 激光物理与工程 激光物理与工程
背景情况:
- 多光子显微镜需要先进的激光系统来进行高分辨率成像.
- 现有的系统可能是重的,复杂的,需要高激发力.
- 需要多功能,紧,低功率的激光源,以实现更广泛的可访问性.
研究的目的:
- 为推出一种新的,紧的,全纤维激光系统,用于先进的成像技术.
- 为了证明其在显著减少的激发功率下进行多光子成像的能力.
- 为了提高实验灵活性和整合在各种显微镜设置.
主要方法:
- 一个增益管理的非线性 (GMN) 放大Yb:光纤振荡器的开发.
- 集成的脉冲选择器可调节的重复率 (0.3-15 MHz).
- 超短脉冲生成 (39 fs) 和峰值功率 (0.83 MW) 的表征.
主要成果:
- 激光系统提供高峰功率的超短脉冲.
- 能够在低至66μW的激发功率下进行多光子成像.
- 证明与双光子,三光子和第二子代显微镜的兼容性.
结论:
- 紧的全纤维激光系统为先进显微镜提供了多功能和高效的解决方案.
- 低刺激功率要求使其适用于敏感的生物样本.
- 该系统的设计简化了集成,并扩大了生物光子学中的应用.
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