饮食中的白醇通过促进与线粒体功能相关的基因表达来改善精液质量,在老年猪中表达
Jing Lv1, Wentao Zhang1, Ruizhi Hu1
1College of Animal Science and Technology, Yuelushan Laboratory, Hunan Agricultural University, Changsha 410128, China.
Journal of animal science
|May 5, 2025
概括
饮食中补充的白醇 (RES) 通过增强精子运动性和生存能力,改善了老化猪精液质量. 这种抗氧化作用与的增加和线粒体功能的改善有关,减少氧化应激.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 氧化压力研究研究 氧化压力研究
背景情况:
- 野猪精液质量对于猪的繁殖效率至关重要.
- 老龄化可能会对野猪的生育能力产生负面影响.
- 在之前的研究中,复星 (RES) 显示出降低精子死亡率的前景.
研究的目的:
- 研究食补充剂对老猪精液质量的影响.
- 为了确定是否可再生能源可以缓解与年龄相关的猪生育率下降.
主要方法:
- 一项为期12周的饮食试验,在老年野猪 (n=6) 中补充了RES (500 mg/kg).
- 血清参数的分析,包括丸激素,皮质醇和甲酸 (MDA).
- 在精液中使用免疫光和电子显微镜评估氧化损伤标记物 (ROS,8-OHDG,MDA).
- 评估线粒体功能相关的基因表达 (PGC-1α,TFB1M,TFB2M) 和线粒体膜潜力 (MMP).
主要成果:
- 补充RES显著延迟了精液质量下降,增加了精子运动性和生存能力 (P < 0.01).
- RES提高了丸激素水平和总抗氧化能力 (P < 0.01),同时降低了血清中的皮质醇 (P < 0.05) 和MDA (P < 0.01).
- RES降低了精液中的活性氧物种 (ROS),8-基-2'-脱氧氨酸 (8-OHDG) 和MDA,减轻了氧化损伤.
- 通过上调调节PGC-1α,TFB1M和TFB2MmRNA表达 (P < 0.01),RES改善了线粒体结构完整性和线粒体膜潜力 (MMP) (P < 0.01).
结论:
- 饮食中的白醇可以提高老猪的精液质量和抗氧化能力.
- RES有可能改善精子线粒体功能,从而减轻与年龄相关的生育能力下降.
- 复星是一种有前途的补充剂,可以提高野猪的生殖效率.
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