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相关概念视频

Alternative RNA Splicing02:18

Alternative RNA Splicing

20.8K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
20.8K
RNA Splicing01:32

RNA Splicing

55.7K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
55.7K
What is Gene Expression?01:36

What is Gene Expression?

8.3K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
8.3K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.8K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.8K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

843
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
843
Master Transcription Regulators02:23

Master Transcription Regulators

6.7K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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相关实验视频

Updated: May 9, 2025

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

12.9K

关于MRAS:主监管机构对替代分离的分析

Lei Zhou1,2,3, Yue Huang1,2,3, Yang Zhao1,2

  • 1China National Center for Bioinformation, Beijing, 100101, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|May 5, 2025
PubMed
概括
此摘要是机器生成的。

一种新的计算方法 - - 替代拼接的主调节器分析 (MRAS) - - 确定了驱动癌症的关键拼接因素. 该工具有助于理解拼接失调及其在瘤发育和变异性中的作用.

关键词:
这是一种RNA结合蛋白.替代性拼接是一种替代性的拼接.主监管机构分析拼接监管网络 拼接监管网络

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

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相关实验视频

Last Updated: May 9, 2025

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

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科学领域:

  • 分子生物学分子生物学
  • 计算生物学 计算生物学
  • 在瘤学瘤学.

背景情况:

  • 拼接失调是癌症的标志,通常是由遗传突变和改变的拼接因素驱动的.
  • 尽管它很普遍,但在固体瘤中确定关键的调控拼接因子仍然具有挑战性.
  • 异常的剪接因子表达有助于瘤的开始,进展,转移和治疗抵抗.

研究的目的:

  • 引入MRAS (替代拼接的主调节器分析),一种用于识别关键拼接因素的计算方法.
  • 证明MRAS能够精确地确定塑造拼接网络并影响细胞过程的主拼接调节器的能力.
  • 发现细胞类型特定的拼接程序和调控关系.

主要方法:

  • 开发MRAS,一种用于分析替代拼接数据的计算方法.
  • 应用MRAS来识别各种癌症表型中的主拼接调节器.
  • 利用单细胞RNA-seq数据上的MRAS揭示细胞类型特定的拼接调节.

主要成果:

  • MRAS成功地确定了与各种癌症表型相关的主拼接调节器,包括发病,进展,转移和治疗耐药性.
  • 分析揭示了控制细胞类型特定拼接程序的关键调控关系.
  • 在剖析拼接监管机制方面,MRAS表现出了准确性和多功能性.

结论:

  • MRAS提供了一种有效和准确的方法来识别癌症中的关键拼接因素.
  • 该工具有助于更深入地了解在瘤发生过程中支失调的基础分子机制.
  • 在批量和单细胞数据中,MRAS是一种用于解开复杂拼接监管网络的多功能方法.