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亲自做 De Novo 抗体测序工作流程,实现可变区域的完全准确性

Meng-Ting He^1,2,3, Ning Li^2,3, Jian-Hua Wang⁴

¹College of Life Sciences, Beijing Normal University, 19 Xinjiekouwai Avenue, Beijing 100875, China.

Journal of proteome research

|May 5, 2025

概括

这项研究提出了一个质谱工作流程,用于准确的抗体测序. 该方法精确地确定了可变区域,使得复合抗体的产生具有与商业版本相同的性能.

科学领域:

生物化学生物化学
免疫学免疫学免疫学
蛋白质组学是指蛋白质组学

背景情况:

作为研究工具和治疗方法,抗体至关重要.
再组合抗体的产生需要对可变区域序列的知识.
基于质谱的de novo测序是确定抗体序列的关键方法.

研究的目的:

为准确的单克隆抗体可变区域测序开发和验证工作流程.
使用质谱数据和开源软件来证明工作流的实用性.

主要方法:

使用基于质谱的新序列测量.
开发了一个工作流程,结合了自由可用的软件工具.
使用自制的抗FLAG单克隆抗体和新型抗HA抗体验证了工作流.

主要成果:

在确定抗体可变区域序列方面实现了100%的准确性.
成功地区分了白氨酸 (L) 和异氨酸 (I).
产生了一种复合抗HA抗体,其性能与商业抗体相匹配.

结论:

描述的工作流程可以实现准确和高效的抗体测序.
这种方法有助于产生具有所需特征的重组抗体.
工作流是抗体研究和开发的宝贵工具.

关键词:

抗HA抗体 (TANA2) 是一种抗HA抗体.在 de novo 测序中使用 de novo 测序.基于免费软件的管道.打开-p 找到

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Next-generation Sequencing

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RNA-seq

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