甲酸对人类支气管细胞在空气液体界面中的影响
Ellie S Burns1, Nicole S Olgun1,2, Sherri A Friend1
1Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia, United States of America.
甲酸 (PAA) 暴露会损害人类支气管细胞,增加细胞毒性和炎症. 更高的PAA度和更长的恢复时间导致更大的细胞死亡和呼吸道损伤.
科学领域:
- * 毒理学 毒理学 毒理学
- * 呼吸系统医学 呼吸系统医学
- * * 职业健康 职业健康 职业健康
背景情况:
- *酸 (PAA) 是一种广泛使用的消毒剂,具有环保的分解产品.
- * 职业接触PAA可能会引起呼吸道刺激,并作为喘因子.
- *了解PAA对人类呼吸道的影响对于职业安全至关重要.
研究的目的:
- * 研究 peracetic acid (PAA) 蒸汽对正常人类支气管上皮细胞 (NHBE) 的毒理作用.
- * 评估PAA暴露后的细胞反应,活力,完整性和炎症标志物.
- * 确定PAA对气道上皮的剂量依赖和时间依赖的影响.
主要方法:
- *在空气液体接口培养的NHBE细胞被暴露在0,3,12,24 ppmPAA中4小时.
- *在暴露后 (4小时和24小时恢复) 评估了细胞活力,细胞毒性和体电阻 (TEER).
- *通过ELISA测量了内甲素-1 (ET-1),IL-6,IL-8并检查了组织学变化.
主要成果:
- *PAA暴露显著增加了细胞毒性,并以剂量和时间依赖的方式降低了细胞活力.
- * 经皮质电阻 (TEER) 在PAA暴露时显著下降.
- *观察到ET-1,IL-6和IL-8的水平升高,在较高度下,组织学证据表明受伤和细胞死亡.
结论:
- *PAA诱导显著的细胞损伤和人类支气管细胞的炎症反应.
- *增加PAA度和恢复时间加剧细胞损伤和死亡率.
- *这些发现突显了与PAA暴露相关的呼吸风险,需要进一步对鼻上皮进行研究.
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