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开发一种LC-MS方法,用于在肌肉组织中测定simvastatin及其氧酸形式以及方法的应用

Ewa Paszkowska¹, Karolina Pietrowska², Steen Larsen³

¹Medical University of Lublin, Department of Bioanalytics, Lublin, Poland.

|May 5, 2025

概括

一种新的分析方法准确地测量肌肉组织中的simvastatin (SIM) 和其代谢物 (SIMA). 这一工具有助于了解他类药物诱导的肌肉副作用.

科学领域:

药理学药理学是指药理学的学科.
分析化学分析化学
生物化学生物化学

背景情况:

类药物广泛用于预防心血管疾病.
类固醇药物治疗可能会导致与肌肉相关的副作用,其机制尚不清楚.
辛巴斯塔丁 (SIM) 和其代谢物辛巴斯塔丁酸 (SIMA) 是人们感兴趣的关键化合物.

研究的目的:

开发和验证一种分析方法,用于在人体肌肉组织中量化simvastatin (SIM) 和simvastatin酸 (SIMA).
为研究他类药物诱导肌肉病变的生物化学基础提供一种工具.

主要方法:

用氨酸缓冲剂对肌肉样本进行均质化.
使用甲醇-乙醇混合物提取他类药物.
通过多重反应监测模式的液体色谱-质谱分析.

主要成果:

该方法证明了线性 (0.1-5 ng/mL),精度 (RSD 6.0-12.9%) 和准确性 (91.4-115.4%).
提取回收率很高 (SIM的76%,SIMA的99%).
该方法成功地应用于接受他类药物治疗的患者的骨肌肉样本.

结论:

开发的方法是确定肌肉组织中SIM和SIMA水平的可靠工具.
这种测试可以有助于理解与他类药物相关的肌肉效应背后的机制.

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