囊素纳米颗粒结合的p54蛋白增强了对非洲猪瘟病毒的免疫反应
Yue Zhang1, Yi Ru1, Longhe Zhao1
1State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
概括
使用非洲猪瘟病毒 (ASFV) p54蛋白与费里丁和封装素纳米颗粒 (F/E-p54) 结合的新型纳米颗粒疫苗的开发显著增强了针对ASFV的免疫反应和抗体产生.
科学领域:
- 兽医免疫学 兽医免疫学
- 疫苗开发 疫苗开发
- 分子病毒学分子病毒学
背景情况:
- 非洲猪瘟病毒 (ASFV) 对全球猪生产构成重大威胁,缺乏有效的商业疫苗.
- ASFV p54蛋白对于病毒进入和细胞内贩运至关重要,与宿主因素相互作用以促进病毒传播.
- 目前的ASFV疫苗策略在引起持久和保护性免疫反应方面面临挑战.
研究的目的:
- 开发和评估一种使用ASFV p54蛋白质的基于纳米粒子的新型疫苗平台.
- 使用SpyTag/SpyCatcher技术增强ASFV的抗原呈现和免疫识别,用于纳米粒子结合.
- 评估ASFV p54-结合纳米颗粒的免疫性和保护功效.
主要方法:
- 通过SpyTag/SpyCatcher系统将ASFV p54蛋白与费里丁和封装素纳米粒子 (F/E-p54) 相结合.
- 评估纳米粒子结合亲和力和向向淋巴细胞组织的向传递.
- 评估B细胞和T细胞的反应,包括T毛囊辅助细胞 (Tfh) 和生殖中心B细胞 (GCB) 的激活.
- 测量中和抗体的产生和ASFV菌株的体外抑制.
主要成果:
- 与可溶性p54.54相比,F/E-p54纳米颗粒显示受体结合亲和力增加了60倍.
- 纳米粒子结合增强了对淋巴细胞组织的向传递,并刺激了强大的Tfh和GCB细胞反应.
- 基于封装素的纳米粒子疫苗引起了强烈,长时间的B细胞和T细胞激活,并产生了中和抗体.
- 生成的抗体有效地抑制了基因型II和复合I/IIASFV菌株.
结论:
- ASFV p54蛋白的纳米粒子结合显著改善了抗原呈现和免疫性.
- 基于囊素的纳米粒子疫苗是一个有希望的平台,可以引起针对ASFV的强大和持续的免疫反应.
- 这种方法有可能促进ASFV疫苗开发和未来的免疫策略.
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