在年轻和中年老年小鼠之间,在上扩张期间,中侧 suture 骨头重塑的比较
Hyeran Helen Jeon1, Mary Cruz Contreras Salas1, Kyungjoon Park2
1Department of Orthodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Bone
|May 5, 2025
概括
年龄会影响正牙治疗的结果. 年轻小鼠在上扩张后表现出强大的骨愈合,而老年小鼠表现出愈合减少和细胞死亡增加,表明需要特定年龄的协议.
科学领域:
- 矯正牙科 矯正牙科是一種矯正牙科.
- 生物医学工程 生物医学工程
- 再生医学是一种再生医学.
背景情况:
- 大扩张可以治疗横向缺陷,但年龄会影响细胞对正牙力量的反应.
- 了解与年龄有关的差异对于制定量身定制的治疗策略至关重要.
研究的目的:
- 为了比较老年相关的细胞对机械力的反应,在大扩张在小鼠.
- 为了研究衰老对骨重塑和介质干细胞 (MSC) 扩张后行为的影响.
主要方法:
- 在6周和12个月大的小鼠身上,使用0.012英寸的. 不钢电线 (25g力).
- 分析包括微CT,TRAP染色和RUNX2,ALP,Gli1,Ki67和TUNEL测定在0,3,7和14天的免疫光.
- 评估的参数是线宽度,骨质细胞活性,骨形成和MSC增殖/亡.
主要成果:
- 两个年龄组都实现了中侧部口,但年轻小鼠表现得更早,骨质细胞活性更强,RUNX2/ALP表达更高.
- 年轻小鼠有更多的Gli1+Ki67+细胞 (增殖),而老年小鼠在第3天有更多的Gli1+TUNEL+细胞 (亡).
- 衰老减少了骨质细胞形成,骨质生成和MSC增殖,同时增加了MSC亡.
结论:
- 衰老会对大扩张期间机械力诱导的骨重塑产生负面影响.
- 骨质细胞和骨质细胞活动减少,以及MSC行为改变,有助于治疗反应的年龄相关差异.
- 这些发现支持需要针对大扩张的特定年龄的正牙治疗方案.
相关概念视频
01:40Bone Remodeling
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
01:22Sutures of the Skull
The human skull is composed of several bones that come together to protect the brain and support the structures of the face. The junctions where these bones meet are called sutures.
Sutures are immobile joints between adjacent bones of the skull. The narrow gap between the bones is filled with dense, fibrous connective tissue that unites the bones. The long sutures located between the skull bones are not straight but instead follow irregular, tightly twisting paths. These twisting lines tightly...
Sutures are immobile joints between adjacent bones of the skull. The narrow gap between the bones is filled with dense, fibrous connective tissue that unites the bones. The long sutures located between the skull bones are not straight but instead follow irregular, tightly twisting paths. These twisting lines tightly...
01:09Changes in the Appendicular Skeleton with Age
The upper and lower limb initially develops as a small bulge called a limb bud, which appears on the lateral side of the early embryo. The upper limb bud appears near the end of the fourth week of development, with the lower limb bud appearing shortly after.
Initially, the limb buds consist of a core of mesenchyme covered by a layer of ectoderm. The ectoderm at the end of the limb bud thickens to form a narrow crest called the apical ectodermal ridge. This ridge stimulates the underlying...
Initially, the limb buds consist of a core of mesenchyme covered by a layer of ectoderm. The ectoderm at the end of the limb bud thickens to form a narrow crest called the apical ectodermal ridge. This ridge stimulates the underlying...
01:09Spongy Bone
All bones comprise an outer layer of compact bone, and an interior made up of spongy bone tissue, also called cancellous or trabecular bone. In long bones, spongy bone tissue is mainly found in the interior of the epiphyses (broad ends of the bone).
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...