相关实验视频
Updated: May 13, 2025
08:56
Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats
Published on: April 7, 2023
802
雌激素增加了Setdb1的细胞质局部化,以稳定Serpinh1并改善骨质母细胞中的蛋白质稳定性
Chunqing Han1, Peiwen Wang2, Junxing Ye3
1MOE Medical Basic Research Innovation Center for Gut Microbiota and Chronic Diseases, Wuxi School of Medicine, Jiangnan University, China; Lab of Modern Environmental Toxicology, School of Public Health Research, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, China.
Molecular and cellular endocrinology
|May 5, 2025
概括
雌激素是一种雌激素.
科学领域:
- 分子生物学分子生物学
- 内分泌学 在内分泌学.
- 骨生物学 骨生物学 骨生物学
背景情况:
- 雌激素通过调节骨质母细胞活动,在骨健康中发挥关键作用.
- Setdb1是一种表观遗传调节器,对维持骨平衡至关重要.
- Setdb1在骨质母细胞中的特定作用和亚细胞局部还不清楚.
研究的目的:
- 为了研究Setdb1在骨质母细胞中的亚细胞分布.
- 阐明细胞质Setdb1在雌激素介导的骨调节中的功能.
- 了解Setdb1影响骨质细胞平衡的机制.
主要方法:
- 免疫光染色和免疫涂抹以分析Setdb1局部化.
- 在骨质细胞细胞系中的基因淘汰 (Atf7ip) 和药理抑制 (Setdb1抑制剂,LMB).
- 卵巢切除 (OVX) 的小鼠模型来评估骨微观结构.
- 蛋白质组分析以确定Setdb1的结合伙伴.
主要成果:
- 通过增强核Setdb1的稳定性,β-雌激醇治疗增加了细胞质Setdb1.
- 降低ATF7ip促进了细胞质Setdb1的局部化,而抑制则降低了它.
- 在骨质母细胞中缺乏Atf7ip的OVX小鼠表现出改善的骨微观结构.
- 发现细胞质Setdb1通过结合和稳定Serpinh1,一个亲原折叠调节器来调节蛋白质平衡.
- 由于未折叠的蛋白质积累,细胞质Setdb1的破坏导致了ER压力.
结论:
- 细胞质Setdb1在β-雌激素介导的骨质细胞恒温中发挥着新的作用.
- Setdb1与Serpinh1的相互作用对于维持蛋白质折叠和预防骨质母细胞中ER压力至关重要.
- 这项研究提高了对雌激素在调节骨健康方面的表观遗传机制的理解.
相关概念视频
01:17Hormones and Bone Tissue
2.5K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.5K
01:40Bone Remodeling
38.0K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.0K
01:31Osteoclasts in Bone Remodeling
2.8K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
2.8K
01:29Bone Disorders
3.3K
Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
3.3K
01:17Role of ER in the Secretory Pathway
4.9K
Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
4.9K
01:26Protein Modifications in the RER
4.9K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
4.9K