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Updated: May 14, 2025

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限制单细胞蛋白质组学中蛋白质泄漏的影响

Andrew Leduc1, Yanxin Xu2, Gergana Shipkovenska2

  • 1Departments of Bioengineering, Biology, Chemistry and Chemical Biology, Single Cell Proteomics Center, Northeastern University, Boston, MA, USA. leduc.an@northeastern.edu.

Nature communications
|May 5, 2025
PubMed
概括

在单细胞蛋白质组学样本准备过程中最大限度地减少蛋白质泄漏对于数据质量至关重要. 我们在小鼠气管细胞中发现了蛋白质泄漏的特征,从而能够准确地识别透细胞,从而改善实验结果.

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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