在Acinetobacter baumannii中发现的菌体编码酶将伪氨基酸转化为8-epip伪氨基酸
Nowshin S Sharar1, Andrea Iovine2, Cristina De Castro2
1Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, QLD, Australia.
新发现的 epaA 和 epaB 基因使 Acinetobacter baumannii 能够产生 8-epipseudaminic 酸. 这些基因,在prophages中发现,修改囊多糖,影响囊类型和疗法.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 基因组学就是基因组学.
背景情况:
- 非酸8-epipseudaminic酸最近在Acinetobacter baumannii中被发现.
- 负责将伪氨基酸转化为其8-聚体的基因不在K位点.
研究的目的:
- 在Acinetobacter baumannii中识别负责8-epipseudaminic酸生物合成的基因.
- 调查这些基因在A. baumannii种群中的流行程度和起源.
主要方法:
- 全基因组分析以确定碳水化合物生物合成基因.
- 囊中的多糖的NMR分析以确认酶活性.
- 对A. baumannii和相关的prophages进行全基因组调查.
主要成果:
- 确定了epaA和epaB作为编码酶的基因,这些酶将伪氨基酸转化为它的8-epimer.
- 在17个不同的Caudoviricetes类在各种A. baumannii分离物中发现了epaA和epaB基因.
- 证明了epaA和epaB的存在会改变囊中的多糖.
结论:
- epaA和 epaB 基因,通常由菌体携带,负责 A. baumannii. 的 8-epipseudaminic 酸的产生.
- 这种囊多糖的修改对A. baumannii囊类型有影响.
- 这些发现表明,针对A. baumannii感染的囊向疗法可能是新的点.
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