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Updated: May 12, 2025

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埃斯切里希亚大肠杆菌尼斯尔1917有效地表达了SARS-CoV-2尖端蛋白的RBD域,没有编码子优化

Shanmugaraja Meenakshi^1,2, R Suvetha³, S Ramadevi⁴

¹Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technologies, Kattankulathur, Tamilnadu, 603203, India. srgmeenakshi@gmail.com.

Scientific reports

|May 5, 2025

概括

工程化益生菌外膜囊泡 (OMVs) 显示SARS-CoV-2受体结合域 (RBD) 对于潜在的COVID-19疫苗. 这种新的方法使用本地RBD表达来提高疫苗的输送和疗效.

科学领域:

疫苗学疫苗学疫苗学
微生物生物技术微生物生物技术
免疫学免疫学免疫学

背景情况:

细菌外膜囊泡 (OMV) 由于其固有的辅助性质,是有效的疫苗输送平台.
一种益生菌Escherichia coli Nissle 1917 (EcN) 为基因工程提供了一个安全且经过临床验证的基础.
在OMV上显示病毒抗原是开发针对传染病的亚单元疫苗的策略.

研究的目的:

设计益生菌Escherichia coli Nissle 1917 (EcN) 来产生表膜囊泡 (OMVs),显示SARS-CoV-2的原生受体结合域 (RBD).
评估与标准细菌系统相比,EcN中本地RBD表达的效率.
评估这些工程OMV作为COVID-19疫苗候选人的潜力.

主要方法:

使用Lpp-OmpA系统对EcN进行基因工程,以表达本地SARS-CoV-2 RBD.
细胞分离以确认RBD在细菌膜中的定位.
通过超离心隔离OMVs的隔离.
在OMV上确认RBD的存在,使用西方斑点和免疫光学.

主要成果:

益生菌EcN证明了本地RBD的显著表达,显示了高效的编码子使用.
RBD成功地局部化到工程 EcN 的膜部分.
孤立的OMV在其表面显示RBD,由生物化学和成像分析证实.
工程OMV显示出抗原呈现细胞优先吸收的潜力.

结论:

工程化EcN衍生的OMV在其表面有效地显示本地SARS-CoV-2 RBD.
这种方法为开发新型COVID-19疫苗候选人提供了一个有希望的策略.
益生菌EcN的使用为基于OMV的疫苗开发提供了一个安全有效的平台.

关键词:

细菌外膜囊泡是一种细菌外膜囊泡.这种疫苗是Covid-19疫苗.在 EcN EcN 中.受体结合域是受体的结合域.这就是SARS-CoV-2病毒.

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