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基于物理组织特性的准空间单细胞转录组定义了肝脏中早期衰老相关的利基

Kwon Yong Tak¹, Juyeon Kim^2,3, Myungsun Park¹

¹Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.

|May 5, 2025

概括

衰老会积累衰老的细胞,影响组织. 一种新方法 - - 纤维基底丰富测序 (FiNi-seq) - - 分析了这些细胞及其微环境,揭示了潜在的抗衰老目标.

科学领域:

老年学和细胞生物学.
组织微环境研究研究
奥米克斯技术的技术.

背景情况:

衰老与衰老细胞的积累有关,经常逃脱免疫清除.
衰老细胞在老化组织中的特征和影响尚不清楚.
目前的奥米克斯方法难以分析稀缺的衰老细胞群.

研究的目的:

开发一种方法来丰富和分析老化组织中老化的细胞.
研究衰老中的纤维的细胞和表观遗传景观.
确定预防衰老的新型治疗点.

主要方法:

开发了使用组织物理性质的纤维缩缩序列 (FiNi-seq).
应用FiNi-seq通过单细胞RNA测序和单核ATAC测序 (ATAC-seq) 来分析年轻和老老的小鼠肝脏.
利用酶性消化阻力进行纤维菌的准空间分析.

主要成果:

在老年肝脏中发现产生Wif1和Smoc1的介质细胞,具有衰老的表型.
在纤维化利基体内的早期免疫反应的特征.
FiNi-ATAC-seq在纤维细胞利基细胞中发现了与年龄相关的表观遗传变化.

结论:

FiNi-seq能够对老化微环境进行详细的准空间单细胞分析.
确定了特定的衰老细胞群和衰老肝脏纤维的表观遗传变化.
这些发现为预防衰老提供了潜在的治疗点.

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