Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

2.3K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
2.3K
Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

11.8K
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
11.8K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

76.1K
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
76.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

First observation of the decay Bs0-->Ds-Ds+ and measurement of its branching ratio.

Physical review letters·2008
Same author

Search for chargino-neutralino production in pp collisions at sq rt[s] = 1.96 TeV.

Physical review letters·2008
Same author

Soluble vascular endothelial-cadherin levels correlate with clinical and biological aspects of severe ovarian hyperstimulation syndrome.

Human reproduction (Oxford, England)·2008
Same author

Improved outcome in high-grade aneurysmal subarachnoid hemorrhage by enhancement of endogenous clearance of cisternal blood clots: a prospective study that demonstrates the role of lamina terminalis fenestration combined with modern microsurgical cisternal blood evacuation.

Minimally invasive neurosurgery : MIN·2008
Same author

Fabrication and characterization of plasma processed surfaces with tuned wettability.

Langmuir : the ACS journal of surfaces and colloids·2007
Same author

First measurement of the W-boson mass in run II of the Tevatron.

Physical review letters·2007

相关实验视频

Updated: May 12, 2025

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

2.4K

药物查的目标是TREM2-TYROBP跨膜结合.

M Cobas-Carreño1, A Esteban-Martos1, L Tomas-Gallardo2

  • 1Departamento de Especialidades Quirúrgicas, Bioquímica e Inmunología, Facultad de Medicina, Universidad de Málaga, Málaga, Spain.

Molecular medicine (Cambridge, Mass.)
|May 5, 2025
PubMed
概括

研究人员选了FDA批准的药物,以找到TREM2-TYROBP相互作用的调节剂,这对于阿尔茨海默病 (AD) 风险至关重要. 瓦伦尼克林作为一种潜在的跨膜激动剂出现,为AD治疗提供了一个新的途径.

关键词:
B2H B2H B2H B2H B2H B2H B2H B2H B2H B2H B2H B2H B2H批量检测 批量检测 批量检测在DAP12中,您可以使用DAP12.药物查是指对药物进行查.在TREM2中,我们可以使用TREM2.在TYROBP中,瓦伦尼克林 瓦伦尼克林

更多相关视频

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
10:20

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Published on: November 11, 2016

8.5K
Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay
09:03

Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay

Published on: March 10, 2020

12.0K

相关实验视频

Last Updated: May 12, 2025

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

2.4K
Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
10:20

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Published on: November 11, 2016

8.5K
Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay
09:03

Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay

Published on: March 10, 2020

12.0K

科学领域:

  • 神经科学是一个神经科学.
  • 免疫学 免疫学 免疫学
  • 药理学 药理学是指药理学的学科.

背景情况:

  • TREM2是一种微质受体,与阿尔茨海默氏症 (AD) 病原发生有关.
  • TREM2的功能依赖于其与TYROBP的相互作用,使这种接口成为AD的关键药物标.
  • 在TREM2中发生的突变是发展AD的重要危险因素.

研究的目的:

  • 开发一个高通量选 (HTS) 系统,以识别TREM2-TYROBP相互作用的调节器.
  • 选FDA批准的药物,以检测TREM2-TYROBP的潜在结合修饰剂.
  • 在人类微质细胞系中验证已识别的化合物.

主要方法:

  • 为TREM2-TYROBP相互作用调节器的HTS设计了一种细菌双杂交 (B2H) 系统.
  • 315种FDA批准的药物在试点研究中进行了选.
  • 通过多个过步骤确保了候选特异性.
  • 在HMC3细胞中测试了有前途的候选人,在抗TREM2 mAb刺激时测量了pSYK/SYK比.

主要成果:

  • B2H系统成功地确定了TREM2-TYROBP相互作用的潜在调节器.
  • 戒烟药物瓦伦尼克林被确定为一种潜在的跨膜激动剂.
  • 瓦伦尼克林证明了其在人类微质细胞中调节TREM2-TYROBP相互作用的能力.

结论:

  • 一个新的B2H查管道有效地确定了TREM2-TYROBP相互作用的调节器.
  • 瓦伦尼克林作为TREM2-TYROBP相互作用的跨膜激动剂.
  • 这一发现表明瓦雷尼克林是阿尔茨海默病的潜在治疗候选者.